Detailed information of TA system TA136995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 119148..119686
• Replicon: plasmid unnamed1
• Accession: NZ_CP043044
• Organism: Gluconobacter thailandicus strain HD924

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A5C0A8C0
• Locus tag: FXF46_RS16195
• Protein ID: WP_062022288.1
• Coordinates: 119363..119686 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FXF46_RS16190
• Protein ID: WP_148621311.1
• Coordinates: 119148..119366 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXF46_RS16180	116673..116960	+	288	Protein_112	hypothetical protein	-
FXF46_RS16185	117017..118879	+	1863	WP_148621310.1	ParB N-terminal domain-containing protein	-
FXF46_RS16190	119148..119366	+	219	WP_148621311.1	antitoxin MazE family protein	Antitoxin
FXF46_RS16195	119363..119686	+	324	WP_062022288.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FXF46_RS16200	120252..120876	+	625	Protein_116	IS66 family transposase	-
FXF46_RS16205	121005..123239	+	2235	WP_148621312.1	hypothetical protein	-
FXF46_RS16210	123242..123619	+	378	WP_148621313.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..173039	173039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11436.30 Da
• Isoelectric Point: 10.2947

>T136995 WP_062022288.1 NZ_CP043044:119363-119686 [Gluconobacter thailandicus]
MNRGDFVTVALQGDLGKPRPALIVQADRFETTASVTVLPVTSTLIDAPLLRLSVTPDERNGLRKTSQVMIDKPSTVSRDK
IGPTFGIASDTVMLSVNRALALFLGLA

Nucleotide

• Length: 324 bp

>T136995 NZ_CP043044:119363-119686 [Gluconobacter thailandicus]
ATGAACCGGGGAGACTTCGTTACCGTTGCTCTCCAGGGTGATCTGGGAAAACCCCGTCCCGCGCTAATCGTCCAGGCTGA
CCGCTTCGAAACGACGGCCTCTGTGACCGTACTCCCTGTTACCAGCACGCTCATAGACGCGCCCTTGCTCCGTCTGTCGG
TAACACCGGACGAGCGAAACGGGCTGCGCAAGACGTCTCAGGTGATGATCGACAAGCCCAGCACTGTCTCCCGCGACAAG
ATCGGTCCAACCTTCGGGATCGCCAGCGATACGGTCATGCTCTCCGTCAATCGCGCTTTGGCCCTCTTTTTAGGTCTGGC
CTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8120.19 Da
• Isoelectric Point: 6.9785

>AT136995 WP_148621311.1 NZ_CP043044:119148-119366 [Gluconobacter thailandicus]
MTPVSIRVQKRRDTLRKAGLRPVQIWVPDTRTPGFDAECQRQARLVAEAERHDPALMSFVESAASDLDGWDA

Nucleotide

• Length: 219 bp

>AT136995 NZ_CP056395:4456637-4456888 [Citrobacter freundii]
ATGCCACATATCATTTTAAGCGACACCACCGCGAGCGTGAGTGAGCTAAAAAAGAACCCGATGGCGACTGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAGCCCGCCTTTTATTGTGTTCCCGCTGAGCTTTACGAAAAAATGC
TTGATGCGCTGGACGATCAGCAACTGGTTAAATTAGTCCATGAACGGAGCCAACAGCCACTGGTGGATGTTGATCTGGAC
AGCTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A5C0A8C0