Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 3305235..3305863 Replicon chromosome
Accession NZ_CP043041
Organism Leptospira interrogans serovar Hardjo strain L53

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FWJ33_RS16705 Protein ID WP_000281042.1
Coordinates 3305465..3305863 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag FWJ33_RS16700 Protein ID WP_001192482.1
Coordinates 3305235..3305465 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FWJ33_RS16670 3301228..3301570 + 343 Protein_2728 tyrosine-type recombinase/integrase -
FWJ33_RS16675 3301619..3302641 + 1023 WP_148636814.1 hypothetical protein -
FWJ33_RS16680 3302696..3303223 + 528 WP_002188620.1 hypothetical protein -
FWJ33_RS16685 3303502..3303837 + 336 WP_000669388.1 type II toxin-antitoxin system RelE/ParE family toxin -
FWJ33_RS16690 3303834..3304133 + 300 WP_000769809.1 helix-turn-helix transcriptional regulator -
FWJ33_RS16695 3304254..3304883 + 630 WP_148636816.1 hypothetical protein -
FWJ33_RS16700 3305235..3305465 + 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FWJ33_RS16705 3305465..3305863 + 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FWJ33_RS16710 3305961..3306509 + 549 WP_000479564.1 hypothetical protein -
FWJ33_RS16715 3306651..3307727 + 1077 WP_001170569.1 hypothetical protein -
FWJ33_RS16720 3307728..3308186 + 459 WP_001239006.1 GNAT family N-acetyltransferase -
FWJ33_RS16725 3308403..3309265 - 863 Protein_2739 hypothetical protein -
FWJ33_RS16730 3309776..3310543 - 768 WP_000636953.1 DUF1554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T136984 WP_000281042.1 NZ_CP043041:3305465-3305863 [Leptospira interrogans serovar Hardjo]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T136984 NZ_CP043041:3305465-3305863 [Leptospira interrogans serovar Hardjo]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT136984 WP_001192482.1 NZ_CP043041:3305235-3305465 [Leptospira interrogans serovar Hardjo]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT136984 NZ_CP056394:c28476-28246 [Enterobacter sp. RHBSTW-00593]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGATGG
GAAAAGCAGACCCGGAGTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References