136983

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1032173..1032749 Replicon chromosome
Accession NZ_CP043041
Organism Leptospira interrogans serovar Hardjo strain L53

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FWJ33_RS06560 Protein ID WP_000617906.1
Coordinates 1032408..1032749 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FWJ33_RS06555 Protein ID WP_000844758.1
Coordinates 1032173..1032421 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FWJ33_RS06540 1027824..1029806 + 1983 WP_000836894.1 GAF domain-containing protein -
FWJ33_RS21220 1030448..1030906 - 459 WP_000453267.1 hypothetical protein -
FWJ33_RS06550 1031060..1031356 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
FWJ33_RS06555 1032173..1032421 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FWJ33_RS06560 1032408..1032749 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FWJ33_RS06565 1033844..1034356 + 513 WP_000079224.1 VOC family protein -
FWJ33_RS06570 1035040..1035633 + 594 WP_001226990.1 dihydrofolate reductase family protein -
FWJ33_RS06575 1035705..1036592 + 888 WP_002188401.1 hypothetical protein -
FWJ33_RS06580 1036710..1037456 + 747 WP_000617027.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T136983 WP_000617906.1 NZ_CP043041:1032408-1032749 [Leptospira interrogans serovar Hardjo]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T136983 NZ_CP043041:1032408-1032749 [Leptospira interrogans serovar Hardjo]
ATGATTCGTGGTGAAATTTGGTGGGTAGATTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT136983 WP_000844758.1 NZ_CP043041:1032173-1032421 [Leptospira interrogans serovar Hardjo]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT136983 NZ_CP056393:c4015586-4015320 [Enterobacter sp. RHBSTW-00593]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCTGATT
TATTTAACTGGCTGATGAATCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References