Detailed information of TA system TA136982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 52389..52911
• Replicon: plasmid pXDL-MCR-1.18
• Accession: NZ_CP043036
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: EOV66_RS27165
• Protein ID: WP_000638823.1
• Coordinates: 52389..52670 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: EOV66_RS27170
• Protein ID: WP_000121741.1
• Coordinates: 52660..52911 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS27130	48538..48858	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
EOV66_RS27135	48929..49117	-	189	WP_050567133.1	hypothetical protein	-
EOV66_RS27140	49219..49803	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
EOV66_RS27145	49824..50222	-	399	WP_001153665.1	hypothetical protein	-
EOV66_RS27150	50341..50778	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
EOV66_RS27155	50784..52019	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
EOV66_RS27160	52022..52321	-	300	WP_000835764.1	IncN plasmid KikA protein	-
EOV66_RS27165	52389..52670	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EOV66_RS27170	52660..52911	-	252	WP_000121741.1	hypothetical protein	Antitoxin
EOV66_RS27175	53011..53646	-	636	WP_015059536.1	hypothetical protein	-
EOV66_RS27180	53694..54526	+	833	Protein_63	DNA primase	-
EOV66_RS27185	54740..54964	-	225	WP_000713561.1	EexN family lipoprotein	-
EOV66_RS27190	54973..55617	-	645	WP_001310442.1	conjugal transfer protein TrbJ	-
EOV66_RS27195	55623..56618	-	996	WP_001028541.1	type IV secretion system protein	-
EOV66_RS27200	56622..56879	-	258	WP_000739144.1	hypothetical protein	-
EOV66_RS27205	56876..57178	-	303	WP_001360345.1	hypothetical protein	-
EOV66_RS27210	57159..57416	-	258	WP_024237697.1	hypothetical protein	-
EOV66_RS27215	57449..57661	-	213	WP_039022940.1	hypothetical protein	-
EOV66_RS27605	57672..57842	-	171	WP_000550720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61603	61603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T136982 WP_000638823.1 NZ_CP043036:c52670-52389 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T136982 NZ_CP043036:c52670-52389 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT136982 WP_000121741.1 NZ_CP043036:c52911-52660 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT136982 NZ_CP043036:c52911-52660 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1