Detailed information of TA system TA136981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 8713..9305
• Replicon: plasmid pXDL-MCR-1.18
• Accession: NZ_CP043036
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: EOV66_RS26915
• Protein ID: WP_000513532.1
• Coordinates: 8713..8961 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: EOV66_RS26920
• Protein ID: WP_000681609.1
• Coordinates: 8958..9305 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS26875	3719..4384	-	666	WP_000532148.1	P-loop NTPase	-
EOV66_RS26885	4974..5303	+	330	WP_001542022.1	hypothetical protein	-
EOV66_RS26890	5704..6219	+	516	WP_001025393.1	J domain-containing protein	-
EOV66_RS26895	6323..6865	+	543	WP_001083904.1	hypothetical protein	-
EOV66_RS26900	6862..7116	+	255	WP_001230709.1	hypothetical protein	-
EOV66_RS26905	7113..7355	+	243	WP_000151456.1	hypothetical protein	-
EOV66_RS27395	7576..7794	+	219	WP_154101533.1	hypothetical protein	-
EOV66_RS26910	7926..8219	+	294	WP_000673428.1	hypothetical protein	-
EOV66_RS26915	8713..8961	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
EOV66_RS26920	8958..9305	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EOV66_RS26925	9393..9575	-	183	WP_001428470.1	hypothetical protein	-
EOV66_RS26930	10077..10376	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
EOV66_RS26935	10636..10962	-	327	WP_000206697.1	hypothetical protein	-
EOV66_RS26940	10964..11404	-	441	WP_023223352.1	hypothetical protein	-
EOV66_RS26945	11424..12131	-	708	WP_000108794.1	hypothetical protein	-
EOV66_RS26950	12133..12885	-	753	WP_000797966.1	conjugal transfer protein TraL	-
EOV66_RS26955	12903..13304	-	402	WP_021575837.1	hypothetical protein	-
EOV66_RS26960	13673..14014	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61603	61603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T136981 WP_000513532.1 NZ_CP043036:8713-8961 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T136981 NZ_CP056393:c1032726-1032508 [Enterobacter sp. RHBSTW-00593]
ATGTCTGAAAAACCACTCACAAAAATCGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATAGACACGCTTGAACGTGT
AATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCGGCTGACCACCGTCTTGCTGAAC
TGACCATGAATAAGCTGTATGACAAAATCCCAACGTCCGTGTGGAGATTTGTCCGTTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT136981 WP_000681609.1 NZ_CP043036:8958-9305 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT136981 NZ_CP056393:c1033128-1032754 [Enterobacter sp. RHBSTW-00593]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTAAACGAGTTGATCGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAATGCGCAAGATTTGCAGAAATGGCGTAAATCGGGCAACCGACT
ATTTCGTTGCTTCATCAACGTGAGCAGAGCTAACCCCGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49