Detailed information of TA system TA136980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 6862..7355
• Replicon: plasmid pXDL-MCR-1.18
• Accession: NZ_CP043036
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: EOV66_RS26900
• Protein ID: WP_001230709.1
• Coordinates: 6862..7116 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: EOV66_RS26905
• Protein ID: WP_000151456.1
• Coordinates: 7113..7355 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS26860	2431..2592	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
EOV66_RS26865	2742..3338	+	597	WP_000125165.1	hypothetical protein	-
EOV66_RS27590	3356..3640	-	285	WP_001542021.1	hypothetical protein	-
EOV66_RS26875	3719..4384	-	666	WP_000532148.1	P-loop NTPase	-
EOV66_RS26885	4974..5303	+	330	WP_001542022.1	hypothetical protein	-
EOV66_RS26890	5704..6219	+	516	WP_001025393.1	J domain-containing protein	-
EOV66_RS26895	6323..6865	+	543	WP_001083904.1	hypothetical protein	-
EOV66_RS26900	6862..7116	+	255	WP_001230709.1	hypothetical protein	Toxin
EOV66_RS26905	7113..7355	+	243	WP_000151456.1	hypothetical protein	Antitoxin
EOV66_RS27395	7576..7794	+	219	WP_154101533.1	hypothetical protein	-
EOV66_RS26910	7926..8219	+	294	WP_000673428.1	hypothetical protein	-
EOV66_RS26915	8713..8961	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	-
EOV66_RS26920	8958..9305	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	-
EOV66_RS26925	9393..9575	-	183	WP_001428470.1	hypothetical protein	-
EOV66_RS26930	10077..10376	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
EOV66_RS26935	10636..10962	-	327	WP_000206697.1	hypothetical protein	-
EOV66_RS26940	10964..11404	-	441	WP_023223352.1	hypothetical protein	-
EOV66_RS26945	11424..12131	-	708	WP_000108794.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61603	61603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-105)

Antitoxin

• Antitoxin CbeA (32-130)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T136980 WP_001230709.1 NZ_CP043036:6862-7116 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T136980 NZ_CP043036:6862-7116 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT136980 WP_000151456.1 NZ_CP043036:7113-7355 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT136980 NZ_CP043036:7113-7355 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5