Detailed information of TA system TA136979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 85150..86773
• Replicon: plasmid pXDL-CTX-M-14
• Accession: NZ_CP043034
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: EOV66_RS25305
• Protein ID: WP_086722013.1
• Coordinates: 85448..86773 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: EOV66_RS25300
• Protein ID: WP_024136327.1
• Coordinates: 85150..85443 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS25270	81022..82263	+	1242	WP_001053338.1	VWA domain-containing protein	-
EOV66_RS25275	82826..83107	-	282	WP_000077926.1	hypothetical protein	-
EOV66_RS25280	83157..83348	-	192	WP_000398480.1	hypothetical protein	-
EOV66_RS25285	83440..83781	-	342	WP_001151575.1	hypothetical protein	-
EOV66_RS25290	84154..84546	+	393	WP_000341066.1	hypothetical protein	-
EOV66_RS25300	85150..85443	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
EOV66_RS25305	85448..86773	+	1326	WP_086722013.1	type II toxin-antitoxin system HipA family toxin	Toxin
EOV66_RS25310	86834..87040	+	207	WP_000134171.1	hypothetical protein	-
EOV66_RS25315	87142..87552	+	411	WP_000985911.1	hypothetical protein	-
EOV66_RS25320	87565..88380	+	816	WP_001572439.1	HNH endonuclease	-
EOV66_RS25325	88634..89059	+	426	WP_001043843.1	hypothetical protein	-
EOV66_RS25330	89513..89749	+	237	Protein_86	hypothetical protein	-
EOV66_RS25335	89804..90103	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	fosA3 / blaCTX-M-14 / aph(3')-Ia / dfrA12 / sul1 / oqxA / oqxB	-	1..224961	224961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50263.22 Da
• Isoelectric Point: 6.1932

>T136979 WP_086722013.1 NZ_CP043034:85448-86773 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEERWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T136979 NZ_CP043034:85448-86773 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGAACGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT136979 WP_024136327.1 NZ_CP043034:85150-85443 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT136979 NZ_CP056393:469821-470063 [Enterobacter sp. RHBSTW-00593]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTTGAAAAACTCAATATCACGCC
AACGGAAGCCGTTCGGTTACTGTTTCAGTACGTTGCGGAGACGGGGCGTATGCCGGTGAAAATGGTCACTGTTAGCGACA
GTGAAGATGAACTCATACAGACAGTCCGGGAGCGGCTGGCTAATCCGCAAAAAGGAATTAAGGTTAGGCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1