Detailed information of TA system TA136976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4455466..4456061
• Replicon: chromosome
• Accession: NZ_CP043033
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: EOV66_RS21615
• Protein ID: WP_000239581.1
• Coordinates: 4455466..4455816 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: EOV66_RS21620
• Protein ID: WP_001223213.1
• Coordinates: 4455810..4456061 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS21595	4450920..4451942	-	1023	WP_012311695.1	ABC transporter permease	-
EOV66_RS21600	4451956..4453458	-	1503	WP_001095664.1	sugar ABC transporter ATP-binding protein	-
EOV66_RS21605	4453591..4454547	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
EOV66_RS21610	4454857..4455387	+	531	WP_000055075.1	inorganic diphosphatase	-
EOV66_RS21615	4455466..4455816	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
EOV66_RS21620	4455810..4456061	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
EOV66_RS21625	4456273..4456614	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
EOV66_RS21630	4456617..4460396	-	3780	WP_000060872.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T136976 WP_000239581.1 NZ_CP043033:c4455816-4455466 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T136976 NZ_CP056386:23462-23878 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT136976 WP_001223213.1 NZ_CP043033:c4456061-4455810 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT136976 NZ_CP043033:c4456061-4455810 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7