136974

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3747441..3748278 Replicon chromosome
Accession NZ_CP043033
Organism Escherichia coli strain XDL

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag EOV66_RS18255 Protein ID WP_000227787.1
Coordinates 3747736..3748278 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag EOV66_RS18250 Protein ID WP_001353405.1
Coordinates 3747441..3747752 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EOV66_RS18225 3742461..3743408 + 948 WP_001239436.1 cytochrome o ubiquinol oxidase subunit II -
EOV66_RS18230 3743430..3745421 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
EOV66_RS18235 3745411..3746025 + 615 WP_151577574.1 cytochrome o ubiquinol oxidase subunit III -
EOV66_RS18240 3746025..3746354 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EOV66_RS18245 3746366..3747256 + 891 WP_000971336.1 heme o synthase -
EOV66_RS18250 3747441..3747752 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
EOV66_RS18255 3747736..3748278 + 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
EOV66_RS18260 3748334..3749269 - 936 WP_012311701.1 sel1 repeat family protein -
EOV66_RS18265 3749676..3751040 + 1365 WP_001000978.1 MFS transporter -
EOV66_RS18270 3751168..3751659 - 492 WP_001138904.1 nucleotide binding protein YajQ -
EOV66_RS18275 3751827..3752738 + 912 WP_000705871.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T136974 WP_000227787.1 NZ_CP043033:3747736-3748278 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T136974 NZ_CP056386:c12939-12634 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT136974 WP_001353405.1 NZ_CP043033:3747441-3747752 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT136974 NZ_CP043033:3747441-3747752 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGCCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References