Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2477500..2478138 Replicon chromosome
Accession NZ_CP043033
Organism Escherichia coli strain XDL

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EOV66_RS11845 Protein ID WP_000813794.1
Coordinates 2477500..2477676 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EOV66_RS11850 Protein ID WP_001270286.1
Coordinates 2477722..2478138 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EOV66_RS11825 2473413..2474333 - 921 Protein_2274 BenE family transporter YdcO -
EOV66_RS11830 2474386..2474922 + 537 WP_000429154.1 DNA-binding transcriptional regulator SutR -
EOV66_RS11835 2474995..2476956 + 1962 WP_012311648.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EOV66_RS11840 2477048..2477278 - 231 WP_000491567.1 DUF2554 family protein -
EOV66_RS11845 2477500..2477676 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EOV66_RS11850 2477722..2478138 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EOV66_RS11855 2478217..2479623 + 1407 WP_000760611.1 PLP-dependent aminotransferase family protein -
EOV66_RS11860 2479868..2481013 + 1146 WP_000034364.1 ABC transporter substrate-binding protein -
EOV66_RS11865 2481031..2482044 + 1014 WP_000220391.1 ABC transporter ATP-binding protein -
EOV66_RS11870 2482045..2482986 + 942 WP_001251303.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2472070..2473398 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T136963 WP_000813794.1 NZ_CP043033:2477500-2477676 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T136963 NZ_CP043033:2477500-2477676 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT136963 WP_001270286.1 NZ_CP043033:2477722-2478138 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT136963 NZ_CP043033:2477722-2478138 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References