Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2154509..2155293 Replicon chromosome
Accession NZ_CP043033
Organism Escherichia coli strain XDL

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag EOV66_RS10275 Protein ID WP_000613626.1
Coordinates 2154509..2155003 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag EOV66_RS10280 Protein ID WP_001110446.1
Coordinates 2155000..2155293 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EOV66_RS10255 2149702..2150799 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
EOV66_RS10260 2150799..2151740 + 942 WP_012311956.1 flagellar assembly peptidoglycan hydrolase FlgJ -
EOV66_RS10265 2151806..2153449 + 1644 WP_000096448.1 flagellar hook-associated protein FlgK -
EOV66_RS10270 2153461..2154414 + 954 WP_001212763.1 flagellar hook-associated protein FlgL -
EOV66_RS10275 2154509..2155003 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
EOV66_RS10280 2155000..2155293 - 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
EOV66_RS10285 2155427..2158600 - 3174 WP_023141578.1 ribonuclease E -
EOV66_RS10290 2159173..2160132 + 960 WP_000846337.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T136958 WP_000613626.1 NZ_CP043033:c2155003-2154509 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T136958 NZ_CP043033:c2155003-2154509 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT136958 WP_001110446.1 NZ_CP043033:c2155293-2155000 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT136958 NZ_CP043033:c2155293-2155000 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References