Detailed information of TA system TA136957

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2106280..2107120
• Replicon: chromosome
• Accession: NZ_CP043033
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: EOV66_RS09970
• Protein ID: WP_000854686.1
• Coordinates: 2106737..2107120 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0YJ34
• Locus tag: EOV66_RS09965
• Protein ID: WP_001360163.1
• Coordinates: 2106280..2106660 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS09940	2103396..2104214	+	819	WP_001234732.1	DUF945 domain-containing protein	-
EOV66_RS09945	2104306..2104791	+	486	WP_000214307.1	antirestriction protein	-
EOV66_RS09950	2104807..2105283	+	477	WP_001186726.1	RadC family protein	-
EOV66_RS09955	2105346..2105567	+	222	WP_000692298.1	DUF987 domain-containing protein	-
EOV66_RS09960	2105586..2106269	+	684	WP_000086769.1	hypothetical protein	-
EOV66_RS09965	2106280..2106660	+	381	WP_001360163.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EOV66_RS09970	2106737..2107120	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
EOV66_RS09975	2107117..2107605	+	489	WP_001054233.1	hypothetical protein	-
EOV66_RS09980	2107622..2107819	+	198	WP_000839253.1	DUF957 domain-containing protein	-
EOV66_RS09985	2107904..2108746	+	843	WP_001274547.1	DUF4942 domain-containing protein	-
EOV66_RS09995	2109217..2109489	+	273	Protein_1917	glyoxylate/hydroxypyruvate reductase GhrA	-
EOV66_RS10005	2109523..2110491	+	969	WP_000654812.1	IS5-like element IS903B family transposase	-
EOV66_RS10010	2110547..2111221	+	675	Protein_1919	glyoxylate/hydroxypyruvate reductase GhrA	-
EOV66_RS10015	2111276..2112013	+	738	WP_000283664.1	zinc-binding phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	2075918..2119815	43897

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T136957 WP_000854686.1 NZ_CP043033:2106737-2107120 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T136957 NZ_CP056372:4264567-4264944 [Klebsiella pneumoniae]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13950.74 Da
• Isoelectric Point: 5.0823

>AT136957 WP_001360163.1 NZ_CP043033:2106280-2106660 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCIVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT136957 NZ_CP056372:4264190-4264516 [Klebsiella pneumoniae]
ATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTTTGGCGCACGGCTGGTGCAGTCGGGCAACTGTCT
GCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACGGGGATTTACGGCATCTCGACCAGGCATTTCCAC
TGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACTCCCCGCCATCAGCACTGTGTCACGCTCTATGCA
AAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGTATACCTGGCGATTTATCCGACACCAGCGGCAAC
CGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1M0YJ34