Detailed information of TA system TA136952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 374241..374884
• Replicon: chromosome
• Accession: NZ_CP043033
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EOV66_RS01735
• Protein ID: WP_049038273.1
• Coordinates: 374241..374528 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: EOV66_RS01740
• Protein ID: WP_000593555.1
• Coordinates: 374525..374884 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS01720	369258..370325	+	1068	WP_000361481.1	HlyD family efflux transporter periplasmic adaptor subunit	-
EOV66_RS01725	370322..373057	+	2736	WP_000149118.1	ribosome-associated ATPase/putative transporter RbbA	-
EOV66_RS01730	373057..374181	+	1125	WP_001314210.1	ABC transporter permease	-
EOV66_RS01735	374241..374528	+	288	WP_049038273.1	type II toxin-antitoxin system HicA family toxin	Toxin
EOV66_RS01740	374525..374884	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EOV66_RS01745	374934..375794	-	861	WP_001131792.1	class II aldolase	-
EOV66_RS01750	375826..376095	-	270	WP_000949581.1	HPr family phosphocarrier protein	-
EOV66_RS01755	376085..377593	-	1509	WP_012311797.1	carbohydrate kinase	-
EOV66_RS01760	377586..378944	-	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
EOV66_RS01765	379018..379299	-	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
EOV66_RS01770	379296..379769	-	474	WP_012311717.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (34-84)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10490.98 Da
• Isoelectric Point: 7.7845

>T136952 WP_049038273.1 NZ_CP043033:374241-374528 [Escherichia coli]
IRTCHENPGTGFTEETKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGA
VESVRDWLLSIGVKP

Nucleotide

• Length: 288 bp

>T136952 NZ_CP043033:374241-374528 [Escherichia coli]
ATAAGGACATGCCATGAGAACCCTGGTACAGGCTTTACGGAAGAAACAAAAAATACATTGGATCAAATATTTAAATCTCC
TGTTCCTCAAGGGATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTT
CGCGTTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCT
GTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT136952 WP_000593555.1 NZ_CP043033:374525-374884 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT136952 NZ_CP056372:358474-358773 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6