Detailed information of TA system TA136935

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 822992..823795
• Replicon: chromosome
• Accession: NZ_CP043009
• Organism: Citrobacter portucalensis strain WCHCP085039

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6TYH6
• Locus tag: FXN82_RS04705
• Protein ID: WP_047358707.1
• Coordinates: 822992..823372 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7L6TXP6
• Locus tag: FXN82_RS04710
• Protein ID: WP_047358708.1
• Coordinates: 823430..823795 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXN82_RS04675	818552..818722	+	171	WP_148361486.1	hypothetical protein	-
FXN82_RS04685	819557..820339	-	783	WP_148361487.1	hypothetical protein	-
FXN82_RS04690	821334..822182	-	849	WP_047358705.1	DUF4942 domain-containing protein	-
FXN82_RS04700	822504..822995	-	492	WP_047358706.1	hypothetical protein	-
FXN82_RS04705	822992..823372	-	381	WP_047358707.1	TA system toxin CbtA family protein	Toxin
FXN82_RS04710	823430..823795	-	366	WP_047358708.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FXN82_RS04715	823817..824038	-	222	WP_047358709.1	DUF987 domain-containing protein	-
FXN82_RS04720	824052..824531	-	480	WP_047358710.1	DNA repair protein RadC	-
FXN82_RS04725	824544..825017	-	474	WP_047358733.1	antirestriction protein	-
FXN82_RS04735	825286..826104	-	819	WP_047358711.1	DUF945 domain-containing protein	-
FXN82_RS04740	826222..826473	-	252	WP_047358712.1	DUF905 family protein	-
FXN82_RS04745	826664..827131	-	468	WP_047358713.1	hypothetical protein	-
FXN82_RS04750	827228..827629	-	402	WP_047358714.1	hypothetical protein	-
FXN82_RS04755	827835..828722	-	888	WP_047358715.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	813057..853689	40632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14216.38 Da
• Isoelectric Point: 10.0929

>T136935 WP_047358707.1 NZ_CP043009:c823372-822992 [Citrobacter portucalensis]
MQTLSAIPTRKAPSHPTPVEIWQQLLTYLLKRHYGLSLSDTQFSDEKIITQYIDAGISLSDALNFLVEKSELVRIDRPGF
SIKHQSPFIGVIDILRARRATGLMQRNGYKRITLLIAGNAAQEQHS

Nucleotide

• Length: 381 bp

>T136935 NZ_CP043009:c823372-822992 [Citrobacter portucalensis]
ATGCAGACCTTATCCGCCATCCCGACGCGGAAGGCACCGTCACACCCGACGCCCGTTGAAATCTGGCAGCAACTTCTTAC
GTATCTGTTAAAAAGACATTACGGCCTTTCGCTTAGCGACACGCAATTTTCCGATGAGAAGATTATTACGCAATATATTG
ACGCCGGTATCTCACTGTCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTGCGGATTGATCGTCCCGGTTTC
TCCATTAAGCATCAGTCCCCTTTTATCGGGGTGATTGATATTCTCCGGGCCAGACGGGCAACGGGTTTGATGCAACGTAA
CGGGTATAAAAGGATCACTCTTCTCATCGCTGGAAATGCAGCTCAGGAGCAACATTCATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13417.25 Da
• Isoelectric Point: 6.4780

>AT136935 WP_047358708.1 NZ_CP043009:c823795-823430 [Citrobacter portucalensis]
MSNQLPPVNHDVSEPWWGLKPGITPCFGARLVQEGNRLHYLADRASIAGVFSDADLRHLDQAFPALLKQLELMLVSGELN
PRHQHCVTLYAKGLTCEADSLGSHGYIYTAMYPTPDNSITR

Nucleotide

• Length: 366 bp

>AT136935 NZ_CP056329:c4602314-4602048 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTAGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6TYH6

Antitoxin

• AlphaFold DB: A0A7L6TXP6