Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 726667..727346 Replicon chromosome
Accession NZ_CP043009
Organism Citrobacter portucalensis strain WCHCP085039

Toxin (Protein)


Gene name ykfI Uniprot ID A0A7D6Z078
Locus tag FXN82_RS04225 Protein ID WP_048215514.1
Coordinates 727005..727346 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0F6RHY7
Locus tag FXN82_RS04220 Protein ID WP_046495841.1
Coordinates 726667..726984 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FXN82_RS04180 722227..723059 + 833 Protein_687 DUF945 domain-containing protein -
FXN82_RS04185 723261..723965 + 705 WP_103798306.1 WYL domain-containing protein -
FXN82_RS04190 723962..724576 + 615 WP_103798305.1 hypothetical protein -
FXN82_RS04195 724665..725075 + 411 WP_103798304.1 hypothetical protein -
FXN82_RS04200 725153..725389 + 237 WP_046495830.1 DUF905 domain-containing protein -
FXN82_RS04205 725468..725926 + 459 WP_053463660.1 antirestriction protein -
FXN82_RS04210 725942..726418 + 477 WP_046495838.1 RadC family protein -
FXN82_RS04215 726427..726648 + 222 WP_000691995.1 DUF987 domain-containing protein -
FXN82_RS04220 726667..726984 + 318 WP_046495841.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FXN82_RS04225 727005..727346 + 342 WP_048215514.1 TA system toxin CbtA family protein Toxin
FXN82_RS04230 727462..728295 + 834 WP_148361457.1 DUF4942 domain-containing protein -
FXN82_RS04240 728599..729105 + 507 WP_148361458.1 G/U mismatch-specific DNA glycosylase -
FXN82_RS04245 729151..730998 - 1848 WP_003024699.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 686516..729105 42589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(2-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13014.97 Da        Isoelectric Point: 10.4182

>T136934 WP_048215514.1 NZ_CP043009:727005-727346 [Citrobacter portucalensis]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T136934 NZ_CP043009:727005-727346 [Citrobacter portucalensis]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11869.49 Da        Isoelectric Point: 6.6245

>AT136934 WP_046495841.1 NZ_CP043009:726667-726984 [Citrobacter portucalensis]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Download         Length: 318 bp

>AT136934 NZ_CP043009:726667-726984 [Citrobacter portucalensis]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6Z078

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RHY7

References