Detailed information of TA system TA136934

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 726667..727346
• Replicon: chromosome
• Accession: NZ_CP043009
• Organism: Citrobacter portucalensis strain WCHCP085039

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7D6Z078
• Locus tag: FXN82_RS04225
• Protein ID: WP_048215514.1
• Coordinates: 727005..727346 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0F6RHY7
• Locus tag: FXN82_RS04220
• Protein ID: WP_046495841.1
• Coordinates: 726667..726984 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXN82_RS04180	722227..723059	+	833	Protein_687	DUF945 domain-containing protein	-
FXN82_RS04185	723261..723965	+	705	WP_103798306.1	WYL domain-containing protein	-
FXN82_RS04190	723962..724576	+	615	WP_103798305.1	hypothetical protein	-
FXN82_RS04195	724665..725075	+	411	WP_103798304.1	hypothetical protein	-
FXN82_RS04200	725153..725389	+	237	WP_046495830.1	DUF905 domain-containing protein	-
FXN82_RS04205	725468..725926	+	459	WP_053463660.1	antirestriction protein	-
FXN82_RS04210	725942..726418	+	477	WP_046495838.1	RadC family protein	-
FXN82_RS04215	726427..726648	+	222	WP_000691995.1	DUF987 domain-containing protein	-
FXN82_RS04220	726667..726984	+	318	WP_046495841.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FXN82_RS04225	727005..727346	+	342	WP_048215514.1	TA system toxin CbtA family protein	Toxin
FXN82_RS04230	727462..728295	+	834	WP_148361457.1	DUF4942 domain-containing protein	-
FXN82_RS04240	728599..729105	+	507	WP_148361458.1	G/U mismatch-specific DNA glycosylase	-
FXN82_RS04245	729151..730998	-	1848	WP_003024699.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	686516..729105	42589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13014.97 Da
• Isoelectric Point: 10.4182

>T136934 WP_048215514.1 NZ_CP043009:727005-727346 [Citrobacter portucalensis]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T136934 NZ_CP043009:727005-727346 [Citrobacter portucalensis]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11869.49 Da
• Isoelectric Point: 6.6245

>AT136934 WP_046495841.1 NZ_CP043009:726667-726984 [Citrobacter portucalensis]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Nucleotide

• Length: 318 bp

>AT136934 NZ_CP043009:726667-726984 [Citrobacter portucalensis]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Structures

Toxin

• AlphaFold DB: A0A7D6Z078

Antitoxin

• AlphaFold DB: A0A0F6RHY7