Detailed information of TA system TA136893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4883830..4884290
• Replicon: chromosome
• Accession: NZ_CP042997
• Organism: Aquisphaera giovannonii strain OJF2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OJF2_RS17740
• Protein ID: WP_148594933.1
• Coordinates: 4884063..4884290 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: OJF2_RS17735
• Protein ID: WP_148594932.1
• Coordinates: 4883830..4884066 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OJF2_RS17705	4879383..4879625	-	243	WP_148594927.1	hypothetical protein	-
OJF2_RS17710	4879704..4880720	-	1017	WP_168221880.1	alpha-L-fucosidase	-
OJF2_RS17715	4880995..4881240	+	246	WP_148594929.1	hypothetical protein	-
OJF2_RS17720	4881237..4881461	+	225	WP_148594930.1	hypothetical protein	-
OJF2_RS17725	4881578..4883029	-	1452	WP_148590767.1	ISKra4 family transposase	-
OJF2_RS17730	4883154..4883384	-	231	WP_148594931.1	hypothetical protein	-
OJF2_RS17735	4883830..4884066	+	237	WP_148594932.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
OJF2_RS17740	4884063..4884290	+	228	WP_148594933.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OJF2_RS17745	4884567..4885013	+	447	WP_148594934.1	hypothetical protein	-
OJF2_RS17750	4885180..4885749	-	570	WP_148594935.1	hypothetical protein	-
OJF2_RS17755	4885951..4886277	+	327	WP_148594936.1	YnfA family protein	-
OJF2_RS17760	4886360..4886794	+	435	WP_148594937.1	TIGR03067 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-59)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 7923.88 Da
• Isoelectric Point: 11.6681

>T136893 WP_148594933.1 NZ_CP042997:4884063-4884290 [Aquisphaera giovannonii]
VSTPASPDRGHLDWLTLDPQAGHEQAGRRPALVLSPASYNQAVGLALFCPVTRQGQAARPSVLSHRSRGPQRSAG

Nucleotide

• Length: 228 bp

>T136893 NZ_CP056301:3977154-3977372 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATTCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 7908.12 Da
• Isoelectric Point: 4.2690

>AT136893 WP_148594932.1 NZ_CP042997:4883830-4884066 [Aquisphaera giovannonii]
MRIRVEKCPGGIALVVPGPLAAQVGLREGEAAELDVTGGQLVVRPGSPATLAELLAGVTPENCHPEWADGPPAGAEML

Nucleotide

• Length: 237 bp

>AT136893 NZ_CP042997:4883830-4884066 [Aquisphaera giovannonii]
ATGCGTATCCGCGTCGAGAAGTGCCCGGGAGGGATTGCGCTGGTCGTGCCGGGACCGCTCGCGGCCCAGGTCGGTCTGCG
CGAGGGTGAGGCGGCCGAGCTGGACGTGACCGGCGGGCAGCTCGTCGTCCGGCCCGGCAGCCCGGCTACGCTCGCGGAGC
TGCTAGCCGGCGTCACGCCCGAGAACTGCCATCCGGAGTGGGCCGACGGCCCGCCTGCCGGGGCCGAGATGCTGTGA