Detailed information of TA system TA136848

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3086627..3087428
• Replicon: chromosome
• Accession: NZ_CP042980
• Organism: Shigella flexneri Y strain PE577

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: FW259_RS16045
• Protein ID: WP_001094430.1
• Coordinates: 3087051..3087428 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FW259_RS16040
• Protein ID: WP_001285622.1
• Coordinates: 3086627..3087004 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FW259_RS16000	3083248..3083703	+	456	WP_005051844.1	hypothetical protein	-
FW259_RS23640	3083804..3084013	+	210	Protein_3042	DUF905 family protein	-
FW259_RS16015	3084114..3084932	+	819	WP_001177758.1	DUF945 domain-containing protein	-
FW259_RS23745	3084932..3085179	+	248	Protein_3044	antirestriction protein	-
FW259_RS16025	3085274..3085747	+	474	WP_000855059.1	antirestriction protein	-
FW259_RS16030	3085763..3086239	+	477	WP_001387238.1	RadC family protein	-
FW259_RS16035	3086326..3086547	+	222	WP_000692350.1	DUF987 domain-containing protein	-
FW259_RS16040	3086627..3087004	+	378	WP_001285622.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FW259_RS16045	3087051..3087428	+	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
FW259_RS16050	3087425..3087913	+	489	WP_000761715.1	hypothetical protein	-
FW259_RS16055	3087933..3088130	+	198	WP_000772029.1	DUF957 domain-containing protein	-
FW259_RS16060	3088215..3089057	+	843	WP_001290187.1	DUF4942 domain-containing protein	-
FW259_RS16065	3089834..3090079	+	246	WP_000875412.1	hypothetical protein	-
FW259_RS16070	3090482..3091644	+	1163	WP_094081514.1	IS3 family transposase	-
FW259_RS16075	3091748..3092215	-	468	WP_011069513.1	prepilin peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T136848 WP_001094430.1 NZ_CP042980:3087051-3087428 [Shigella flexneri Y]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T136848 NZ_CP042980:3087051-3087428 [Shigella flexneri Y]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13697.54 Da
• Isoelectric Point: 6.3127

>AT136848 WP_001285622.1 NZ_CP042980:3086627-3087004 [Shigella flexneri Y]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLNQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT136848 NZ_CP042980:3086627-3087004 [Shigella flexneri Y]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGAACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7