Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1547785..1548311 Replicon chromosome
Accession NZ_CP042980
Organism Shigella flexneri Y strain PE577

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FW259_RS08065 Protein ID WP_000323025.1
Coordinates 1548024..1548311 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FW259_RS08060 Protein ID WP_000534858.1
Coordinates 1547785..1548024 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FW259_RS08040 1545060..1545809 - 750 Protein_1537 fimbrial protein -
FW259_RS08045 1545865..1546562 + 698 Protein_1538 IS1 family transposase -
FW259_RS08050 1546580..1547458 + 879 Protein_1539 IS3-like element IS600 family transposase -
FW259_RS08055 1547455..1547760 - 306 WP_094108346.1 hypothetical protein -
FW259_RS08060 1547785..1548024 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FW259_RS08065 1548024..1548311 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FW259_RS08070 1548383..1548538 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FW259_RS08075 1548756..1549007 + 252 WP_000980987.1 hypothetical protein -
FW259_RS08080 1549074..1549352 + 279 Protein_1545 hypothetical protein -
FW259_RS08085 1549354..1550403 + 1050 WP_001265249.1 DUF968 domain-containing protein -
FW259_RS08090 1550416..1550772 + 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
FW259_RS08095 1550787..1551608 + 822 WP_000762882.1 antitermination protein -
FW259_RS08115 1552495..1552626 + 132 Protein_1549 DUF3927 family protein -
FW259_RS08120 1552907..1553242 - 336 WP_000871291.1 anti-adapter protein IraM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1537331..1564393 27062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T136836 WP_000323025.1 NZ_CP042980:1548024-1548311 [Shigella flexneri Y]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T136836 NZ_CP042980:1548024-1548311 [Shigella flexneri Y]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT136836 WP_000534858.1 NZ_CP042980:1547785-1548024 [Shigella flexneri Y]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT136836 NZ_CP042980:1547785-1548024 [Shigella flexneri Y]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References