Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1775564..1776154 Replicon chromosome
Accession NZ_CP042977
Organism Klebsiella pneumoniae strain KPN55602

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag FVE92_RS10465 Protein ID WP_022615588.1
Coordinates 1775822..1776154 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag FVE92_RS10460 Protein ID WP_000288812.1
Coordinates 1775564..1775821 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE92_RS10440 1771899..1773647 + 1749 WP_023302666.1 hypothetical protein -
FVE92_RS10445 1773726..1774282 + 557 Protein_1667 hypothetical protein -
FVE92_RS10450 1774565..1775026 + 462 WP_004213450.1 hypothetical protein -
FVE92_RS10455 1775023..1775229 + 207 WP_022615589.1 helix-turn-helix domain-containing protein -
FVE92_RS10460 1775564..1775821 + 258 WP_000288812.1 antitoxin Antitoxin
FVE92_RS10465 1775822..1776154 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FVE92_RS10480 1776476..1777912 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
FVE92_RS10490 1778278..1779732 - 1455 WP_004148975.1 AMP nucleosidase -
FVE92_RS10495 1779862..1780107 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T136815 WP_022615588.1 NZ_CP042977:1775822-1776154 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T136815 NZ_CP042977:1775822-1776154 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT136815 WP_000288812.1 NZ_CP042977:1775564-1775821 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT136815 NZ_CP056245:247080-247439 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTTAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References