Detailed information of TA system TA136812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 39797..40379
• Replicon: plasmid pK55602_2
• Accession: NZ_CP042976
• Organism: Klebsiella pneumoniae strain KPN55602

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: E8PSG2
• Locus tag: FVE92_RS01720
• Protein ID: WP_013583078.1
• Coordinates: 40083..40379 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A223LLE5
• Locus tag: FVE92_RS01715
• Protein ID: WP_058998984.1
• Coordinates: 39797..40081 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE92_RS01680	35309..35602	-	294	WP_058998996.1	TrbC/VirB2 family protein	-
FVE92_RS01685	35620..36249	-	630	WP_058998994.1	lytic transglycosylase domain-containing protein	-
FVE92_RS01690	36256..36453	-	198	WP_058998992.1	hypothetical protein	-
FVE92_RS01695	36443..36937	-	495	WP_058998990.1	hypothetical protein	-
FVE92_RS01700	37344..37736	+	393	WP_080398147.1	helix-turn-helix domain-containing protein	-
FVE92_RS01705	37733..38902	-	1170	WP_058998988.1	hypothetical protein	-
FVE92_RS01710	38906..39451	-	546	WP_058998986.1	hypothetical protein	-
FVE92_RS01715	39797..40081	-	285	WP_058998984.1	putative addiction module antidote protein	Antitoxin
FVE92_RS01720	40083..40379	-	297	WP_013583078.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FVE92_RS01725	40718..40855	-	138	WP_161400388.1	hypothetical protein	-
FVE92_RS01730	40902..41114	-	213	WP_058998981.1	hypothetical protein	-
FVE92_RS01735	41520..41753	-	234	WP_072223385.1	hypothetical protein	-
FVE92_RS01740	41743..42000	-	258	WP_058998978.1	hypothetical protein	-
FVE92_RS01745	41997..42539	-	543	WP_058998977.1	hypothetical protein	-
FVE92_RS01750	42543..42743	-	201	WP_167840815.1	hypothetical protein	-
FVE92_RS01755	42755..42970	-	216	WP_058998973.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..43296	43296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11290.10 Da
• Isoelectric Point: 10.3166

>T136812 WP_013583078.1 NZ_CP042976:c40379-40083 [Klebsiella pneumoniae]
MIEIKRTPEVEKWLRSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRIHQGKGYRVYFGNKNNQIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Nucleotide

• Length: 297 bp

>T136812 NZ_CP042976:c40379-40083 [Klebsiella pneumoniae]
ATGATAGAAATAAAACGGACGCCGGAAGTTGAAAAGTGGCTCAGGTCTTTGAAGGACAGAACGGCAAAGGCAAAAATATT
GATGCGACTTGAGCGCATGGAGGACGGAAATTTTGGAGACGTGGAGCCAATAGGTGACGGATTATCAGAATTAAGGATTC
ATCAGGGTAAAGGCTACCGTGTGTATTTTGGAAACAAAAACAATCAAATTATTCTCTTGCTTTGTGGTGGAGATAAAAGC
ACGCAGCAAGCAGATATCAGAAAAGCGAAACAATTAGCTAAACAGTGGGGGTTTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10557.97 Da
• Isoelectric Point: 4.7254

>AT136812 WP_058998984.1 NZ_CP042976:c40081-39797 [Klebsiella pneumoniae]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNISQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Nucleotide

• Length: 285 bp

>AT136812 NZ_CP042976:c40081-39797 [Klebsiella pneumoniae]
ATGAAAGAAGAACTTAAAAAATTTGATGTTGTTGAATTTCTTGAAGATGAAGAAGATATTCAGGAATATCTTAATGCGGC
AATTGAAGAAGATGATACAAAATATCTTTTTAAAGCATTAGGTAACATAGCCAGAGCAAGAAACATAAGTCAGCTATCTA
AAGAAGTTGGTATGTCCAGGGAGGGAATTTACAAGGCGTTATCCGGTGAAGGAAATCCATCATTCCAGACTGTCTCTAAA
ATCACAAAAGCACTTGGTTTAAAACTTCACTTCACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A223LLP6

Antitoxin

• AlphaFold DB: A0A223LLE5