Detailed information of TA system TA136809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 144574..145310
• Replicon: plasmid pK55602_1
• Accession: NZ_CP042975
• Organism: Klebsiella pneumoniae strain KPN55602

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FVE92_RS00770
• Protein ID: WP_004213601.1
• Coordinates: 144574..145056 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: FVE92_RS00775
• Protein ID: WP_004213599.1
• Coordinates: 145044..145310 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE92_RS00750	139928..141274	+	1347	WP_011154555.1	dihydroorotase	-
FVE92_RS00755	141338..142360	+	1023	WP_004214536.1	porphobilinogen synthase	-
FVE92_RS00760	142535..142930	+	396	Protein_153	DDE-type integrase/transposase/recombinase	-
FVE92_RS00765	142926..144153	-	1228	Protein_154	IS3 family transposase	-
FVE92_RS00770	144574..145056	-	483	WP_004213601.1	GNAT family N-acetyltransferase	Toxin
FVE92_RS00775	145044..145310	-	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
FVE92_RS00780	145605..146432	-	828	WP_087846572.1	APH(6) family putative aminoglycoside O-phosphotransferase	-
FVE92_RS00785	146429..147073	-	645	WP_087846574.1	recombinase family protein	-
FVE92_RS00790	147343..148266	-	924	WP_114435917.1	LysR family transcriptional regulator	-
FVE92_RS00795	148391..149164	+	774	WP_087846578.1	SDR family oxidoreductase	-
FVE92_RS00800	149250..149516	+	267	WP_114268457.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	rmpA / rmpA / iroN / iroD / iroC / iroB	1..270776	270776
	-	inside	IScluster/Tn	-	-	142926..147073	4147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17307.01 Da
• Isoelectric Point: 10.0704

>T136809 WP_004213601.1 NZ_CP042975:c145056-144574 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETALDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T136809 NZ_CP042975:c145056-144574 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGCCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT136809 WP_004213599.1 NZ_CP042975:c145310-145044 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT136809 NZ_CP056244:c1950416-1950183 [Citrobacter freundii]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGTCCGCGACCAAAGTGGCC
TGAAAAAAAGGACGATTGTCACCCGCTAGAATGCAAAAACCGCAAGTTCTGCAGCCAGAACGTTGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A7D3T1D0