Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 44031..44632 Replicon plasmid pCFSAN061768
Accession NZ_CP042974
Organism Escherichia coli strain CFSAN061768

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CA694_RS23815 Protein ID WP_001216034.1
Coordinates 44031..44411 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CA694_RS23820 Protein ID WP_001190712.1
Coordinates 44411..44632 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA694_RS23780 (CA694_024105) 39082..40014 - 933 WP_000081352.1 homocysteine S-methyltransferase -
CA694_RS23785 (CA694_024110) 40001..41404 - 1404 WP_001373486.1 S-methylmethionine permease -
CA694_RS23790 (CA694_024115) 41648..42628 + 981 Protein_44 IS5 family transposase -
CA694_RS23795 (CA694_024120) 42838..43173 + 336 WP_169329198.1 type I deoxyribonuclease HsdR -
CA694_RS23800 (CA694_024125) 43123..43536 - 414 Protein_46 integrase core domain-containing protein -
CA694_RS23805 (CA694_024130) 43541..43819 - 279 Protein_47 pdcB -
CA694_RS23810 (CA694_024135) 43847..44026 - 180 WP_001513661.1 hypothetical protein -
CA694_RS23815 (CA694_024140) 44031..44411 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CA694_RS23820 (CA694_024145) 44411..44632 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CA694_RS23825 (CA694_024150) 44895..45592 + 698 WP_226458177.1 IS1-like element IS1A family transposase -
CA694_RS23830 (CA694_024155) 45779..46072 - 294 WP_226438991.1 biofilm development regulator YmgB/AriR family protein -
CA694_RS23835 (CA694_024160) 46376..46480 - 105 Protein_53 IS3 family transposase -
CA694_RS24425 46507..46656 - 150 Protein_54 IS3 family transposase -
CA694_RS23845 (CA694_024170) 47870..48484 + 615 WP_032214276.1 acid-sensing system DNA-binding response regulator EvgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrS1 / blaCTX-M-15 / blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..117296 117296
- inside IScluster/Tn - - 37048..47302 10254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T136807 WP_001216034.1 NZ_CP042974:c44411-44031 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T136807 NZ_CP056244:c325480-325163 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAGGGATTTAGTAAACGCCGTCCGTTACTTTTACCTGACGATGAGTTTCGTGCCTTTCA
AGAGGCGCTTATTGAAAACCCTGAGGCAGGGGATACTATCGCTGGTACGGGGGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGTAAGCGCAGCGGTATTCGGGTTATCTATTACAACGTGACGCGTAAAGGTCGAATCTATCTGGCTTTGCTC
TATCCTAAGAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCGATATGCTGATTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT136807 WP_001190712.1 NZ_CP042974:c44632-44411 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT136807 NZ_CP042974:c44632-44411 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References