Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4459715..4460212 Replicon chromosome
Accession NZ_CP042973
Organism Escherichia coli strain CFSAN061768

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CA694_RS21315 Protein ID WP_001173343.1
Coordinates 4459715..4459888 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CA694_RS21320 Protein ID WP_000398619.1
Coordinates 4459916..4460212 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA694_RS21305 (4456385) 4456385..4457842 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
CA694_RS21310 (4458079) 4458079..4459596 + 1518 WP_001550472.1 lysine--tRNA ligase -
CA694_RS21315 (4459715) 4459715..4459888 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CA694_RS21320 (4459916) 4459916..4460212 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CA694_RS21325 (4460441) 4460441..4460713 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
CA694_RS21330 (4460725) 4460725..4460955 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
CA694_RS21335 (4461135) 4461135..4462766 + 1632 WP_001216477.1 sensor histidine kinase -
CA694_RS21340 (4462763) 4462763..4463482 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136805 WP_001173343.1 NZ_CP042973:c4459888-4459715 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136805 NZ_CP042973:c4459888-4459715 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136805 WP_000398619.1 NZ_CP042973:c4460212-4459916 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136805 NZ_CP042973:c4460212-4459916 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References