Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4195771..4196347 Replicon chromosome
Accession NZ_CP042973
Organism Escherichia coli strain CFSAN061768

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag CA694_RS20060 Protein ID WP_001297643.1
Coordinates 4196060..4196347 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag CA694_RS20055 Protein ID WP_000063160.1
Coordinates 4195771..4196073 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA694_RS20040 (4192367) 4192367..4194517 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
CA694_RS20045 (4194567) 4194567..4194770 + 204 WP_000467859.1 YbdD/YjiX family protein -
CA694_RS20050 (4194781) 4194781..4195737 + 957 WP_001297640.1 GTPase -
CA694_RS20055 (4195771) 4195771..4196073 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
CA694_RS20060 (4196060) 4196060..4196347 - 288 WP_001297643.1 BrnT family toxin Toxin
CA694_RS20065 (4196546) 4196546..4197242 - 697 Protein_3941 IS1 family transposase -
CA694_RS20070 (4197557) 4197557..4198789 + 1233 WP_001825915.1 multidrug efflux MFS transporter MdtM -
CA694_RS20075 (4198830) 4198830..4200110 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4196546..4196755 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T136803 WP_001297643.1 NZ_CP042973:c4196347-4196060 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T136803 NZ_CP042973:c4196347-4196060 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT136803 WP_000063160.1 NZ_CP042973:c4196073-4195771 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT136803 NZ_CP056239:143999-144268 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References