Detailed information of TA system TA136799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3232005..3232710
• Replicon: chromosome
• Accession: NZ_CP042973
• Organism: Escherichia coli strain CFSAN061768

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CA694_RS15650
• Protein ID: WP_000539521.1
• Coordinates: 3232005..3232391 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CA694_RS15655
• Protein ID: WP_001280945.1
• Coordinates: 3232381..3232710 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA694_RS15630 (3228009)	3228009..3228635	+	627	WP_001595548.1	glutathione S-transferase GstB	-
CA694_RS15635 (3228632)	3228632..3229747	-	1116	WP_001595547.1	aldose sugar dehydrogenase YliI	-
CA694_RS15640 (3229858)	3229858..3230241	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CA694_RS15645 (3230454)	3230454..3231779	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CA694_RS15650 (3232005)	3232005..3232391	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA694_RS15655 (3232381)	3232381..3232710	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CA694_RS15660 (3232780)	3232780..3233022	-	243	Protein_3085	diguanylate cyclase	-
CA694_RS15665 (3233078)	3233078..3233775	+	698	WP_225893468.1	IS1 family transposase	-
CA694_RS15670 (3233791)	3233791..3234885	-	1095	Protein_3087	diguanylate cyclase	-
CA694_RS15675 (3234893)	3234893..3237241	-	2349	WP_001328220.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T136799 WP_000539521.1 NZ_CP042973:3232005-3232391 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T136799 NZ_CP042973:3232005-3232391 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT136799 WP_001280945.1 NZ_CP042973:3232381-3232710 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT136799 NZ_CP042973:3232381-3232710 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6