Detailed information of TA system TA136797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2582408..2583046
• Replicon: chromosome
• Accession: NZ_CP042973
• Organism: Escherichia coli strain CFSAN061768

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: CA694_RS12435
• Protein ID: WP_000813795.1
• Coordinates: 2582870..2583046 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CA694_RS12430
• Protein ID: WP_076797675.1
• Coordinates: 2582408..2582824 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA694_RS12410 (2577560)	2577560..2578501	-	942	WP_001251315.1	ABC transporter permease	-
CA694_RS12415 (2578502)	2578502..2579515	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
CA694_RS12420 (2579533)	2579533..2580678	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
CA694_RS12425 (2580923)	2580923..2582329	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
CA694_RS12430 (2582408)	2582408..2582824	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CA694_RS12435 (2582870)	2582870..2583046	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CA694_RS12440 (2583268)	2583268..2583498	+	231	WP_023910283.1	YncJ family protein	-
CA694_RS12445 (2583590)	2583590..2585551	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CA694_RS12450 (2585624)	2585624..2586160	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
CA694_RS12455 (2586252)	2586252..2587424	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T136797 WP_000813795.1 NZ_CP042973:c2583046-2582870 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T136797 NZ_CP042973:c2583046-2582870 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT136797 WP_076797675.1 NZ_CP042973:c2582824-2582408 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT136797 NZ_CP042973:c2582824-2582408 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1