Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 918889..919543 Replicon chromosome
Accession NZ_CP042973
Organism Escherichia coli strain CFSAN061768

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CA694_RS04410 Protein ID WP_000244777.1
Coordinates 919136..919543 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CA694_RS04405 Protein ID WP_000354046.1
Coordinates 918889..919155 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA694_RS04380 (914058) 914058..914801 + 744 WP_000951948.1 SDR family oxidoreductase -
CA694_RS04385 (914858) 914858..916291 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
CA694_RS04390 (916336) 916336..916647 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CA694_RS04395 (916811) 916811..917470 + 660 WP_116319264.1 hemolysin III family protein -
CA694_RS04400 (917666) 917666..918646 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CA694_RS04405 (918889) 918889..919155 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CA694_RS04410 (919136) 919136..919543 + 408 WP_000244777.1 protein YgfX Toxin
CA694_RS04415 (919583) 919583..920104 - 522 WP_001055874.1 flavodoxin FldB -
CA694_RS04420 (920216) 920216..921112 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CA694_RS04425 (921137) 921137..921847 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CA694_RS04430 (921853) 921853..923586 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T136783 WP_000244777.1 NZ_CP042973:919136-919543 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T136783 NZ_CP056232:3859859-3860077 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTGGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT136783 WP_000354046.1 NZ_CP042973:918889-919155 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT136783 NZ_CP042973:918889-919155 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References