Detailed information of TA system TA136773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 13453..14045
• Replicon: plasmid pCFSAN061769_01
• Accession: NZ_CP042970
• Organism: Escherichia coli strain CFSAN061769

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: CA697_RS24070
• Protein ID: WP_000520549.1
• Coordinates: 13453..13701 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CA697_RS24075
• Protein ID: WP_116326949.1
• Coordinates: 13698..14045 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA697_RS24040 (CA697_024390)	10330..10845	+	516	WP_001025395.1	J domain-containing protein	-
CA697_RS24045 (CA697_024395)	10950..11492	+	543	WP_001083906.1	hypothetical protein	-
CA697_RS24050 (CA697_024400)	11489..11740	+	252	WP_001230707.1	hypothetical protein	-
CA697_RS24055 (CA697_024405)	11727..11954	+	228	WP_000801440.1	hypothetical protein	-
CA697_RS24060 (CA697_024415)	12408..12707	+	300	WP_000650309.1	hypothetical protein	-
CA697_RS24065 (CA697_024420)	12798..13148	+	351	WP_000854259.1	hypothetical protein	-
CA697_RS24070 (CA697_024425)	13453..13701	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
CA697_RS24075 (CA697_024430)	13698..14045	+	348	WP_116326949.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CA697_RS24080 (CA697_024440)	14406..14909	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
CA697_RS24085 (CA697_024445)	14913..16142	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
CA697_RS24090 (CA697_024450)	16219..16512	+	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
CA697_RS24095 (CA697_024455)	16509..16724	+	216	WP_000760375.1	EexN family lipoprotein	-
CA697_RS24100 (CA697_024460)	17080..17700	+	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
CA697_RS24105 (CA697_024465)	17714..18040	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33304	33304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T136773 WP_000520549.1 NZ_CP042970:13453-13701 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T136773 NZ_CP042970:13453-13701 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13140.91 Da
• Isoelectric Point: 4.8175

>AT136773 WP_116326949.1 NZ_CP042970:13698-14045 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKKSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT136773 NZ_CP042970:13698-14045 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAAAATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3