Detailed information of TA system TA136772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 11489..11954
• Replicon: plasmid pCFSAN061769_01
• Accession: NZ_CP042970
• Organism: Escherichia coli strain CFSAN061769

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: CA697_RS24050
• Protein ID: WP_001230707.1
• Coordinates: 11489..11740 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: CA697_RS24055
• Protein ID: WP_000801440.1
• Coordinates: 11727..11954 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA697_RS24025 (CA697_024375)	6970..7497	-	528	WP_000577043.1	DUF2726 domain-containing protein	-
CA697_RS24030 (CA697_024380)	7632..8036	-	405	WP_012421203.1	hypothetical protein	-
CA697_RS24035 (CA697_024385)	8049..8915	-	867	WP_001353743.1	replication initiation protein	-
CA697_RS24040 (CA697_024390)	10330..10845	+	516	WP_001025395.1	J domain-containing protein	-
CA697_RS24045 (CA697_024395)	10950..11492	+	543	WP_001083906.1	hypothetical protein	-
CA697_RS24050 (CA697_024400)	11489..11740	+	252	WP_001230707.1	hypothetical protein	Toxin
CA697_RS24055 (CA697_024405)	11727..11954	+	228	WP_000801440.1	hypothetical protein	Antitoxin
CA697_RS24060 (CA697_024415)	12408..12707	+	300	WP_000650309.1	hypothetical protein	-
CA697_RS24065 (CA697_024420)	12798..13148	+	351	WP_000854259.1	hypothetical protein	-
CA697_RS24070 (CA697_024425)	13453..13701	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
CA697_RS24075 (CA697_024430)	13698..14045	+	348	WP_116326949.1	type II toxin-antitoxin system HicB family antitoxin	-
CA697_RS24080 (CA697_024440)	14406..14909	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
CA697_RS24085 (CA697_024445)	14913..16142	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
CA697_RS24090 (CA697_024450)	16219..16512	+	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
CA697_RS24095 (CA697_024455)	16509..16724	+	216	WP_000760375.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33304	33304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T136772 WP_001230707.1 NZ_CP042970:11489-11740 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T136772 NZ_CP056229:3554268-3554486 [Citrobacter freundii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGTCTTCGACGCTGCCAGACAATTGACACGCTCGAGCGCGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTGTACGACAAGATTCCAACTTCCGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT136772 WP_000801440.1 NZ_CP042970:11727-11954 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT136772 NZ_CP042970:11727-11954 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9