Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4470699..4471196 Replicon chromosome
Accession NZ_CP042969
Organism Escherichia coli strain CFSAN061769

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CA697_RS21515 Protein ID WP_001173343.1
Coordinates 4470699..4470872 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CA697_RS21520 Protein ID WP_000398619.1
Coordinates 4470900..4471196 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA697_RS21505 (4467369) 4467369..4468826 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
CA697_RS21510 (4469063) 4469063..4470580 + 1518 WP_001295074.1 lysine--tRNA ligase -
CA697_RS21515 (4470699) 4470699..4470872 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CA697_RS21520 (4470900) 4470900..4471196 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CA697_RS21525 (4471423) 4471423..4471695 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
CA697_RS21530 (4471707) 4471707..4471937 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
CA697_RS21535 (4472118) 4472118..4473749 + 1632 WP_001216461.1 sensor histidine kinase -
CA697_RS21540 (4473746) 4473746..4474465 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4400527..4471196 70669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136769 WP_001173343.1 NZ_CP042969:c4470872-4470699 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136769 NZ_CP042969:c4470872-4470699 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136769 WP_000398619.1 NZ_CP042969:c4471196-4470900 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136769 NZ_CP056229:c2361925-2361641 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References