Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3624699..3625536 Replicon chromosome
Accession NZ_CP042969
Organism Escherichia coli strain CFSAN061769

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag CA697_RS17515 Protein ID WP_000227784.1
Coordinates 3624994..3625536 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag CA697_RS17510 Protein ID WP_001297137.1
Coordinates 3624699..3625010 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA697_RS17485 (3619719) 3619719..3620666 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
CA697_RS17490 (3620688) 3620688..3622679 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
CA697_RS17495 (3622669) 3622669..3623283 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
CA697_RS17500 (3623283) 3623283..3623612 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
CA697_RS17505 (3623624) 3623624..3624514 + 891 WP_000971336.1 heme o synthase -
CA697_RS17510 (3624699) 3624699..3625010 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
CA697_RS17515 (3624994) 3624994..3625536 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
CA697_RS17520 (3625592) 3625592..3626527 - 936 WP_032197347.1 tetratricopeptide repeat protein -
CA697_RS17525 (3626935) 3626935..3628299 + 1365 WP_001000978.1 MFS transporter -
CA697_RS17530 (3628427) 3628427..3628918 - 492 WP_001138904.1 nucleotide binding protein YajQ -
CA697_RS17535 (3629086) 3629086..3629997 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T136765 WP_000227784.1 NZ_CP042969:3624994-3625536 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T136765 NZ_CP056229:c318475-318158 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAGGGATTTAGTAAACGCCGTCCGTTACTTTTACCTGACGATGAGTTTCGTGCCTTTCA
AGAGGCGCTTATTGAAAACCCTGAGGCAGGGGATACTATCGCTGGTACGGGGGGATTTAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGTAAGCGCAGCGGTATTCGGGTTATCTATTACAACGTGACGCGTAAAGGTCGAATCTATCTGGCTTTGCTC
TATCCTAAGAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCGATATGCTGATTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT136765 WP_001297137.1 NZ_CP042969:3624699-3625010 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT136765 NZ_CP042969:3624699-3625010 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References