Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1373474..1374099 Replicon chromosome
Accession NZ_CP042969
Organism Escherichia coli strain CFSAN061769

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CA697_RS06595 Protein ID WP_000911329.1
Coordinates 1373701..1374099 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CA697_RS06590 Protein ID WP_000450524.1
Coordinates 1373474..1373701 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA697_RS06565 (1369276) 1369276..1369746 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
CA697_RS06570 (1369746) 1369746..1370318 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CA697_RS06575 (1370464) 1370464..1371342 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CA697_RS06580 (1371359) 1371359..1372393 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CA697_RS06585 (1372606) 1372606..1373319 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CA697_RS06590 (1373474) 1373474..1373701 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CA697_RS06595 (1373701) 1373701..1374099 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
CA697_RS06600 (1374246) 1374246..1375109 + 864 WP_063082385.1 neutral zinc metallopeptidase -
CA697_RS06605 (1375124) 1375124..1377139 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
CA697_RS06610 (1377213) 1377213..1377911 + 699 WP_000679812.1 esterase -
CA697_RS06615 (1377992) 1377992..1378192 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T136751 WP_000911329.1 NZ_CP042969:1373701-1374099 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T136751 NZ_CP042969:1373701-1374099 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT136751 WP_000450524.1 NZ_CP042969:1373474-1373701 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT136751 NZ_CP042969:1373474-1373701 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References