Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 903867..904521 Replicon chromosome
Accession NZ_CP042969
Organism Escherichia coli strain CFSAN061769

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CA697_RS04385 Protein ID WP_000244781.1
Coordinates 904114..904521 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CA697_RS04380 Protein ID WP_000354046.1
Coordinates 903867..904133 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA697_RS04355 (899036) 899036..899779 + 744 WP_089667583.1 SDR family oxidoreductase -
CA697_RS04360 (899836) 899836..901269 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
CA697_RS04365 (901314) 901314..901625 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CA697_RS04370 (901789) 901789..902448 + 660 WP_000250274.1 hemolysin III family protein -
CA697_RS04375 (902644) 902644..903624 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
CA697_RS04380 (903867) 903867..904133 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CA697_RS04385 (904114) 904114..904521 + 408 WP_000244781.1 protein YgfX Toxin
CA697_RS04390 (904561) 904561..905082 - 522 WP_001055874.1 flavodoxin FldB -
CA697_RS04395 (905194) 905194..906090 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CA697_RS04400 (906115) 906115..906825 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CA697_RS04405 (906831) 906831..908564 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T136749 WP_000244781.1 NZ_CP042969:904114-904521 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T136749 NZ_CP042969:904114-904521 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT136749 WP_000354046.1 NZ_CP042969:903867-904133 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT136749 NZ_CP042969:903867-904133 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References