Detailed information of TA system TA136748

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 705926..706767
• Replicon: chromosome
• Accession: NZ_CP042969
• Organism: Escherichia coli strain CFSAN061769

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: CA697_RS03400
• Protein ID: WP_000854822.1
• Coordinates: 706384..706767 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CA697_RS03395
• Protein ID: WP_096959852.1
• Coordinates: 705926..706294 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA697_RS03375 (703671)	703671..704489	+	819	WP_077737897.1	DUF932 domain-containing protein	-
CA697_RS03380 (704580)	704580..705065	+	486	WP_000214317.1	antirestriction protein	-
CA697_RS03385 (705080)	705080..705556	+	477	WP_001186747.1	RadC family protein	-
CA697_RS03390 (705625)	705625..705846	+	222	WP_000692309.1	DUF987 domain-containing protein	-
CA697_RS03395 (705926)	705926..706294	+	369	WP_096959852.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CA697_RS03400 (706384)	706384..706767	+	384	WP_000854822.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
CA697_RS03405 (706758)	706758..707246	+	489	WP_001177592.1	DUF5983 family protein	-
CA697_RS03410 (707258)	707258..707455	+	198	WP_000839263.1	DUF957 domain-containing protein	-
CA697_RS03415 (707552)	707552..708397	+	846	WP_116326960.1	DUF4942 domain-containing protein	-
CA697_RS03425 (708696)	708696..709202	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
CA697_RS03430 (709280)	709280..711121	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T136748 WP_000854822.1 NZ_CP042969:706384-706767 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T136748 NZ_CP042969:706384-706767 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13667.40 Da
• Isoelectric Point: 6.4768

>AT136748 WP_096959852.1 NZ_CP042969:705926-706294 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKR

Nucleotide

• Length: 369 bp

>AT136748 NZ_CP042969:705926-706294 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAGGTAA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1