Detailed information of TA system TA136725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3072452..3073047
• Replicon: chromosome
• Accession: NZ_CP042946
• Organism: Escherichia fergusonii strain ATCC 35470

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: ABH49_RS14830
• Protein ID: WP_000155159.1
• Coordinates: 3072670..3073047 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: ABH49_RS14825
• Protein ID: WP_000557315.1
• Coordinates: 3072452..3072673 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABH49_RS14800	3067528..3068637	+	1110	WP_105283503.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
ABH49_RS14805	3068685..3069611	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ABH49_RS14810	3069608..3070885	+	1278	WP_000803807.1	branched chain amino acid ABC transporter permease LivM	-
ABH49_RS14815	3070882..3071649	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ABH49_RS14820	3071651..3072364	+	714	WP_105283502.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ABH49_RS14825	3072452..3072673	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ABH49_RS14830	3072670..3073047	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ABH49_RS14835	3073256..3074572	+	1317	WP_105283501.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ABH49_RS14840	3074670..3075557	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ABH49_RS14845	3075554..3076399	+	846	WP_000572172.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ABH49_RS14850	3076401..3077471	+	1071	WP_105283500.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3069608..3078211	8603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T136725 WP_000155159.1 NZ_CP042946:3072670-3073047 [Escherichia fergusonii]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T136725 NZ_CP042946:3072670-3073047 [Escherichia fergusonii]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAGGATATCGTTACCCGCTTGCGTCAACGCGGAACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT136725 WP_000557315.1 NZ_CP042946:3072452-3072673 [Escherichia fergusonii]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT136725 NZ_CP042946:3072452-3072673 [Escherichia fergusonii]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTGGTTCTGGAAACCGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32