Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1385111..1385661 Replicon chromosome
Accession NZ_CP042945
Organism Escherichia fergusonii strain ATCC 35471

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag FV159_RS07500 Protein ID WP_001160963.1
Coordinates 1385111..1385425 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag FV159_RS07505 Protein ID WP_000125566.1
Coordinates 1385428..1385661 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FV159_RS07475 1380786..1382072 + 1287 WP_000800490.1 peptidylprolyl isomerase SurA -
FV159_RS07480 1382072..1383061 + 990 WP_000241251.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
FV159_RS07485 1383058..1383879 + 822 WP_001065390.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
FV159_RS07490 1383882..1384259 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
FV159_RS07495 1384266..1385114 + 849 WP_000257179.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
FV159_RS07500 1385111..1385425 - 315 WP_001160963.1 CcdB family protein Toxin
FV159_RS07505 1385428..1385661 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
FV159_RS07510 1385747..1386226 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
FV159_RS07515 1386419..1388281 - 1863 WP_000377099.1 glutathione-regulated potassium-efflux system protein KefC -
FV159_RS07520 1388274..1388804 - 531 WP_000600745.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
FV159_RS07525 1388912..1390243 - 1332 WP_001183186.1 MFS transporter -
FV159_RS07530 1390302..1390589 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T136701 WP_001160963.1 NZ_CP042945:c1385425-1385111 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T136701 NZ_CP042945:c1385425-1385111 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT136701 WP_000125566.1 NZ_CP042945:c1385661-1385428 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT136701 NZ_CP042945:c1385661-1385428 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References