Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1142750..1143333 Replicon chromosome
Accession NZ_CP042945
Organism Escherichia fergusonii strain ATCC 35471

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag FV159_RS06405 Protein ID WP_000254750.1
Coordinates 1142750..1143085 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FV159_RS06410 Protein ID WP_000581937.1
Coordinates 1143085..1143333 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FV159_RS06390 1138637..1139935 - 1299 WP_000036723.1 phosphopyruvate hydratase -
FV159_RS06395 1140023..1141660 - 1638 WP_000210873.1 CTP synthase (glutamine hydrolyzing) -
FV159_RS06400 1141888..1142679 - 792 WP_001071668.1 nucleoside triphosphate pyrophosphohydrolase -
FV159_RS06405 1142750..1143085 - 336 WP_000254750.1 endoribonuclease MazF Toxin
FV159_RS06410 1143085..1143333 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FV159_RS06415 1143411..1145645 - 2235 WP_000226844.1 GTP diphosphokinase -
FV159_RS06420 1145696..1146994 - 1299 WP_000046872.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1137254..1138582 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T136700 WP_000254750.1 NZ_CP042945:c1143085-1142750 [Escherichia fergusonii]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T136700 NZ_CP042945:c1143085-1142750 [Escherichia fergusonii]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGTGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT136700 WP_000581937.1 NZ_CP042945:c1143333-1143085 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT136700 NZ_CP042945:c1143333-1143085 [Escherichia fergusonii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References