Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2330679..2331262 Replicon chromosome
Accession NZ_CP042942
Organism Escherichia fergusonii strain ATCC 35473

Toxin (Protein)


Gene name mazF Uniprot ID B7LWP2
Locus tag FV158_RS11440 Protein ID WP_000254732.1
Coordinates 2330927..2331262 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FV158_RS11435 Protein ID WP_000581937.1
Coordinates 2330679..2330927 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FV158_RS11425 2327018..2328316 + 1299 WP_148048041.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
FV158_RS11430 2328367..2330601 + 2235 WP_000226844.1 GTP diphosphokinase -
FV158_RS11435 2330679..2330927 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FV158_RS11440 2330927..2331262 + 336 WP_000254732.1 endoribonuclease MazF Toxin
FV158_RS11445 2331333..2332124 + 792 WP_001071668.1 nucleoside triphosphate pyrophosphohydrolase -
FV158_RS11450 2332352..2333989 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FV158_RS11455 2334077..2335375 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12156.08 Da        Isoelectric Point: 7.7128

>T136669 WP_000254732.1 NZ_CP042942:2330927-2331262 [Escherichia fergusonii]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSDQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T136669 NZ_CP056168:c4093119-4092832 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCCTTCTTGTTATTTTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT136669 WP_000581937.1 NZ_CP042942:2330679-2330927 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT136669 NZ_CP042942:2330679-2330927 [Escherichia fergusonii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B7LWP2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References