Detailed information of TA system TA136657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 19680..20299
• Replicon: chromosome
• Accession: NZ_CP042942
• Organism: Escherichia fergusonii strain ATCC 35473

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: FV158_RS00105
• Protein ID: WP_001280991.1
• Coordinates: 20081..20299 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: FV158_RS00100
• Protein ID: WP_104917851.1
• Coordinates: 19680..20054 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FV158_RS00090	14812..16005	+	1194	WP_002431407.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
FV158_RS00095	16028..19177	+	3150	WP_001132493.1	multidrug efflux RND transporter permease subunit	-
FV158_RS00100	19680..20054	+	375	WP_104917851.1	Hha toxicity modulator TomB	Antitoxin
FV158_RS00105	20081..20299	+	219	WP_001280991.1	hemolysin expression modulator Hha	Toxin
FV158_RS00110	20796..21266	+	471	WP_002431406.1	YlaC family protein	-
FV158_RS00115	21405..22955	+	1551	WP_046083533.1	EAL domain-containing protein	-
FV158_RS00120	22997..23350	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
FV158_RS00130	23729..24040	+	312	WP_000409907.1	MGMT family protein	-
FV158_RS00135	24071..24643	-	573	WP_000779831.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T136657 WP_001280991.1 NZ_CP042942:20081-20299 [Escherichia fergusonii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T136657 NZ_CP056166:c74107-73709 [Escherichia marmotae]
ATGCTGAAATTCATGCTGGACACGAATATCTGTATCTTCACGATTAAGAACAAACCGGCGCATATTCGGGAACGATTCAA
CCTGAACTCAGGGCGAATGTGCATCAGCTCAGTTACCGTGATGGAGCTGGTATACGGCGCTGAAAAAAGTCAGTTTCCGG
AGCGTAATCTCGCGATTATAGAAGGATTTATTTCGCGCCTTGACGTGCTGGATTACGACTGCGCTGCTGCCACACATACC
GGTCAGATCCGTGCTGAGCTCGCCCGTCAGGGACGTCCGGTGGGACCGTTTGATCAGATGATTGCCGGTCATGCCCGTAG
CCGGGGACTCATTGTGGTCACAAACAATACCCGGGAGTTTGAACGTATCTCCGGCCTTCGTCTGGAAGACTGGTGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.31 Da
• Isoelectric Point: 4.8989

>AT136657 WP_104917851.1 NZ_CP042942:19680-20054 [Escherichia fergusonii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT136657 NZ_CP056166:c74334-74107 [Escherichia marmotae]
ATGGAAACGACCGTATTTCTCAGTAACCGGAGTCAGGCAGTCAGGCTGCCGAAAGCTGTAGCCCTGCCTGAAGACGTAAA
AAAAGTCGACGTTATTGCTGTTGGCCGTACCCGGATCATTACCCCGGCAGGAGAGTCCTGGGACAGCTGGTTCGATGGCG
ACAGTGTCAGCGCAGATTTCATGAATGACAGAGAACAACCCGCCGTACAGGAAAGGGAGTCGTTCTGA

Structures

Toxin

• PDB: 4ICG