Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3602857..3603657 Replicon chromosome
Accession NZ_CP042934
Organism Escherichia coli 042

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag FVF73_RS17640 Protein ID WP_000342449.1
Coordinates 3602857..3603384 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag FVF73_RS17645 Protein ID WP_001277108.1
Coordinates 3603391..3603657 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVF73_RS17615 3597932..3598699 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
FVF73_RS17620 3598696..3599973 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
FVF73_RS17625 3599970..3600896 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
FVF73_RS17630 3600944..3602053 - 1110 WP_089455105.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
FVF73_RS17635 3602477..3602860 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
FVF73_RS17640 3602857..3603384 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
FVF73_RS17645 3603391..3603657 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
FVF73_RS17650 3603807..3604910 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
FVF73_RS17655 3605182..3606036 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
FVF73_RS17660 3606281..3607339 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
FVF73_RS17665 3607332..3608000 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T136637 WP_000342449.1 NZ_CP042934:c3603384-3602857 [Escherichia coli 042]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T136637 NZ_CP056159:c2675094-2674921 [Escherichia marmotae]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTCTCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGCCTGCCCGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT136637 WP_001277108.1 NZ_CP042934:c3603657-3603391 [Escherichia coli 042]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT136637 NZ_CP042934:c3603657-3603391 [Escherichia coli 042]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References