Detailed information of TA system TA136631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2571153..2571778
• Replicon: chromosome
• Accession: NZ_CP042934
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FVF73_RS12500
• Protein ID: WP_000911330.1
• Coordinates: 2571153..2571551 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FVF73_RS12505
• Protein ID: WP_000450524.1
• Coordinates: 2571551..2571778 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVF73_RS12480	2567031..2567231	+	201	WP_000383836.1	YpfN family protein	-
FVF73_RS12485	2567341..2568039	-	699	WP_000679823.1	esterase	-
FVF73_RS12490	2568113..2570128	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FVF73_RS12495	2570143..2571006	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
FVF73_RS12500	2571153..2571551	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FVF73_RS12505	2571551..2571778	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FVF73_RS12510	2571932..2572645	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FVF73_RS12515	2572858..2573892	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FVF73_RS12520	2573909..2574787	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FVF73_RS12525	2574933..2575505	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FVF73_RS12530	2575505..2575975	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T136631 WP_000911330.1 NZ_CP042934:c2571551-2571153 [Escherichia coli 042]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T136631 NZ_CP042934:c2571551-2571153 [Escherichia coli 042]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT136631 WP_000450524.1 NZ_CP042934:c2571778-2571551 [Escherichia coli 042]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT136631 NZ_CP042934:c2571778-2571551 [Escherichia coli 042]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33