Detailed information of TA system TA136616

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3840738..3841507
• Replicon: chromosome
• Accession: NZ_CP042930
• Organism: Leclercia adecarboxylata strain J656

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: FTX45_RS18215
• Protein ID: WP_048213143.1
• Coordinates: 3840738..3841124 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FTX45_RS18220
• Protein ID: WP_177933861.1
• Coordinates: 3841181..3841507 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTX45_RS18180	3835818..3836693	-	876	WP_032613829.1	LysR family transcriptional regulator	-
FTX45_RS18185	3836800..3837261	+	462	WP_059307252.1	multidrug/biocide efflux PACE transporter	-
FTX45_RS18190	3837380..3838609	+	1230	WP_144839549.1	amino acid permease	-
FTX45_RS18195	3838653..3838925	-	273	WP_059307250.1	N-acetyltransferase	-
FTX45_RS18200	3838936..3839166	-	231	WP_032613838.1	(4Fe-4S)-binding protein	-
FTX45_RS18210	3839812..3840651	-	840	WP_048213142.1	DUF4942 domain-containing protein	-
FTX45_RS18215	3840738..3841124	-	387	WP_048213143.1	TA system toxin CbtA family protein	Toxin
FTX45_RS18220	3841181..3841507	-	327	WP_177933861.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FTX45_RS18225	3841565..3841786	-	222	WP_004192297.1	DUF987 domain-containing protein	-
FTX45_RS18230	3841800..3842279	-	480	WP_150872343.1	DNA repair protein RadC	-
FTX45_RS18235	3842291..3842740	-	450	WP_150872345.1	antirestriction protein	-
FTX45_RS18240	3842773..3843594	-	822	WP_150872347.1	DUF945 domain-containing protein	-
FTX45_RS18245	3844334..3844609	+	276	Protein_3523	transposase	-
FTX45_RS18250	3844565..3845653	-	1089	WP_065523308.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (8-110)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14428.55 Da
• Isoelectric Point: 8.9843

>T136616 WP_048213143.1 NZ_CP042930:c3841124-3840738 [Leclercia adecarboxylata]
MQTKLPFMRAASSRPSPVDVWQTLLTCLLEHHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGFS
IMEQSPFITAIDILRARKATGLMNRGTYKEVTAITRGQHPQARTSGKR

Nucleotide

• Length: 387 bp

>T136616 NZ_CP042930:c3841124-3840738 [Leclercia adecarboxylata]
ATGCAAACGAAATTACCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTTGATGTCTGGCAGACTCTCCTGACCTG
CCTGCTGGAGCACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAACAGGTGATTCAGCAGCATATCGATG
CGGGTATCTCGCTGGTCGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTCAGC
ATCATGGAGCAGTCTCCGTTTATTACGGCGATTGATATCCTCCGGGCCAGAAAGGCTACGGGGCTGATGAACCGTGGTAC
GTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCACGCACTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12082.76 Da
• Isoelectric Point: 6.4645

>AT136616 WP_177933861.1 NZ_CP042930:c3841507-3841181 [Leclercia adecarboxylata]
ISEPWWGLRRSVSPCFGARLVQEGNRLYYLADRANVSGQFCDADLRHLDQSFPVLMKQLELMLTSGELNPRHQHGVTLCA
KGLTCEADTLGSHGYVYIAIYPTPVTTE

Nucleotide

• Length: 327 bp

>AT136616 NZ_CP042930:c3841507-3841181 [Leclercia adecarboxylata]
ATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTTGGTGCCCGACTGGTCCAGGAGGGGAACCGCCT
GTATTACCTGGCTGACCGGGCGAATGTTAGCGGACAGTTCTGTGATGCAGATTTGCGCCATCTTGACCAGTCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACGGCGTCACACTGTGTGCA
AAAGGCCTGACCTGCGAGGCCGATACCCTCGGTTCGCATGGCTACGTTTATATCGCGATTTATCCCACTCCCGTCACCAC
AGAATAA