Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1077120..1077739 Replicon chromosome
Accession NZ_CP042930
Organism Leclercia adecarboxylata strain J656

Toxin (Protein)


Gene name Hha Uniprot ID A0A345CDT0
Locus tag FTX45_RS05055 Protein ID WP_032616864.1
Coordinates 1077120..1077338 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3E1ZZ82
Locus tag FTX45_RS05060 Protein ID WP_032616865.1
Coordinates 1077347..1077739 (-) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTX45_RS05035 1073964..1074317 + 354 WP_059308601.1 DUF1428 family protein -
FTX45_RS05040 1074350..1075918 - 1569 WP_103791840.1 EAL domain-containing protein -
FTX45_RS05045 1076184..1076324 + 141 WP_032616862.1 type B 50S ribosomal protein L36 -
FTX45_RS05050 1076384..1076848 - 465 WP_032616863.1 membrane protein -
FTX45_RS05055 1077120..1077338 - 219 WP_032616864.1 hemolysin expression modulator Hha Toxin
FTX45_RS05060 1077347..1077739 - 393 WP_032616865.1 Hha toxicity modulator TomB Antitoxin
FTX45_RS05065 1078230..1081370 - 3141 WP_103791842.1 multidrug efflux RND transporter permease subunit AcrB -
FTX45_RS05070 1081393..1082586 - 1194 WP_150870234.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1066853..1077739 10886


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T136603 WP_032616864.1 NZ_CP042930:c1077338-1077120 [Leclercia adecarboxylata]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Download         Length: 219 bp

>T136603 NZ_CP042930:c1077338-1077120 [Leclercia adecarboxylata]
ATGTCTGATAAACCCTTAACCAAGACAGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATTGACACCCTTGAACGTGT
GATTGAGAAAAATAAATACGAGCTTTCTGATAATGAGTTAGCAGTATTCTATTCTGCAGCCGATCATCGTCTGGCTGAAC
TCACTATGAATAAGCTGTATGATAAAATCCCGACAGCCGTGTGGAAGTTTGTACGCTGA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 15093.98 Da        Isoelectric Point: 4.8941

>AT136603 WP_032616865.1 NZ_CP042930:c1077739-1077347 [Leclercia adecarboxylata]
MDEYSPKRQDIAQLKFLCESLYHDCLSNLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNASKANPLASPVKIITNN

Download         Length: 393 bp

>AT136603 NZ_CP042930:c1077739-1077347 [Leclercia adecarboxylata]
ATGGACGAATACTCGCCTAAAAGGCAAGATATTGCACAGTTGAAATTCCTTTGTGAATCCCTGTACCATGACTGCCTTTC
CAATCTCGATGAAAGCAACCATGGCTGGGTCAATGACCCTACTTCTGCCATTAATTTGCAGTTAAACGAGTTGATTGAGC
ATATAGCAACCTTCGCGCTTAATTATAAAATTAAGTATAACGAAGATAATAAGTTGATTGAGCAAATTGACGAATACCTG
GATGACACTTTTATGTTATTCAGCAGCTACGGCATTAATGCACAGGATTTGCAAAAATGGCGTAAATCGGGCAATCGGCT
TTTTCGTTGTTTTGTCAATGCAAGCAAGGCTAACCCGTTAGCTTCGCCAGTTAAAATTATTACAAACAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A345CDT0

Antitoxin

Source ID Structure
AlphaFold DB A0A3E1ZZ82

References