Detailed information of TA system TA136602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2465153..2466131
• Replicon: chromosome
• Accession: NZ_CP042929
• Organism: Bacillus cereus strain G1-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: FED50_RS14475
• Protein ID: WP_000624977.1
• Coordinates: 2465153..2465890 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: FED50_RS14480
• Protein ID: WP_000237819.1
• Coordinates: 2466003..2466131 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FED50_RS14450	2460766..2461173	+	408	WP_000063587.1	VOC family protein	-
FED50_RS14455	2461197..2461577	+	381	WP_002025805.1	hypothetical protein	-
FED50_RS14460	2461735..2463420	-	1686	WP_088054504.1	alpha-keto acid decarboxylase family protein	-
FED50_RS14465	2463528..2464010	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
FED50_RS14470	2464177..2464914	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FED50_RS14475	2465153..2465890	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
FED50_RS14480	2466003..2466131	+	129	WP_000237819.1	hypothetical protein	Antitoxin
FED50_RS14485	2466204..2466380	+	177	WP_000852629.1	stage II sporulation protein SB	-
FED50_RS14490	2466400..2466789	-	390	Protein_2428	YxeA family protein	-
FED50_RS14495	2467001..2468470	+	1470	WP_065382524.1	beta-Ala-His dipeptidase	-
FED50_RS14500	2468716..2469525	+	810	WP_000678370.1	C39 family peptidase	-
FED50_RS14505	2469551..2470153	+	603	WP_000517271.1	hypothetical protein	-
FED50_RS14510	2470397..2470648	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2433006..2471240	38234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T136602 WP_000624977.1 NZ_CP042929:2465153-2465890 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T136602 NZ_CP056151:23347-23817 [Klebsiella grimontii]
GTGATCCTCTACAGGCTGACGAAAACAAAGAATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAATTGATCCAAAGCGCAAATATGGAT
GAATTACCGGCCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCTCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACTCTGATTTTT
ACGGGATCGTGCAGATGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT136602 WP_000237819.1 NZ_CP042929:2466003-2466131 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT136602 NZ_CP042929:2466003-2466131 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2