Detailed information of TA system TA136600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 438172..438690
• Replicon: plasmid unnamed2
• Accession: NZ_CP042915
• Organism: Rhodococcus qingshengii strain RL1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q6XN60
• Locus tag: D6M20_RS02075
• Protein ID: WP_011133429.1
• Coordinates: 438388..438690 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8E3P5W3
• Locus tag: D6M20_RS02070
• Protein ID: WP_064444958.1
• Coordinates: 438172..438387 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6M20_RS31710	434095..434304	+	210	WP_166503019.1	hypothetical protein	-
D6M20_RS02050	434225..434527	-	303	Protein_407	transposase	-
D6M20_RS02055	434566..435879	-	1314	WP_030538128.1	IS256 family transposase	-
D6M20_RS02060	436017..436799	-	783	WP_021334706.1	DUF4145 domain-containing protein	-
D6M20_RS02065	437141..437860	+	720	WP_021334705.1	hypothetical protein	-
D6M20_RS02070	438172..438387	+	216	WP_064444958.1	hypothetical protein	Antitoxin
D6M20_RS02075	438388..438690	+	303	WP_011133429.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D6M20_RS02080	439292..440689	+	1398	WP_149357541.1	hypothetical protein	-
D6M20_RS02085	440689..441486	+	798	WP_149357431.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..448745	448745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10470.01 Da
• Isoelectric Point: 4.7317

>T136600 WP_011133429.1 NZ_CP042915:438388-438690 [Rhodococcus qingshengii]
VRPIHDAQLGGLCPVLVLTRELVRPHLGTVTVAPIRSEGRGLSTELSLGEGNGVEDGSVVDCDGVTTIPVSALGVNRGFL
LPAQEVLLSRVLRSAFDLES

Nucleotide

• Length: 303 bp

>T136600 NZ_CP042915:438388-438690 [Rhodococcus qingshengii]
GTGCGGCCGATTCACGACGCCCAGCTTGGAGGTCTTTGTCCGGTGTTGGTTCTGACGAGGGAATTGGTCCGGCCTCATCT
GGGGACGGTCACTGTTGCTCCCATTCGTTCGGAGGGGCGGGGGTTGTCGACGGAACTGAGTTTAGGCGAAGGCAACGGCG
TCGAGGACGGATCGGTTGTCGACTGCGACGGGGTGACGACGATCCCGGTGTCGGCACTCGGGGTGAATCGAGGTTTTCTC
TTGCCGGCTCAGGAAGTGTTGTTGAGCAGGGTTCTGCGATCAGCATTCGATCTCGAATCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7670.69 Da
• Isoelectric Point: 8.9167

>AT136600 WP_064444958.1 NZ_CP042915:438172-438387 [Rhodococcus qingshengii]
MSTQIAVRLPEDIVAFLDSEVSGGRAKSRAQVVLRALEREQRRQLAVRDVELLSDGAGSDVNIAAAHGKKN

Nucleotide

• Length: 216 bp

>AT136600 NZ_CP042915:438172-438387 [Rhodococcus qingshengii]
ATGAGCACGCAGATCGCGGTACGGCTACCCGAGGACATCGTGGCGTTTCTCGACAGCGAGGTCAGCGGTGGACGCGCGAA
AAGCCGTGCCCAAGTGGTGCTTCGAGCGTTGGAGCGTGAGCAGCGTCGGCAGCTGGCGGTCCGGGACGTGGAGCTCTTGT
CTGACGGCGCGGGTAGCGATGTGAATATCGCTGCCGCGCACGGAAAGAAGAACTGA

Structures

Toxin

• AlphaFold DB: Q6XN60