136599

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 272649..273214 Replicon plasmid unnamed2
Accession NZ_CP042915
Organism Rhodococcus qingshengii strain RL1

Toxin (Protein)


Gene name mazF Uniprot ID C3JNM9
Locus tag D6M20_RS01340 Protein ID WP_003943396.1
Coordinates 272858..273214 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID C3JNM8
Locus tag D6M20_RS01335 Protein ID WP_003943374.1
Coordinates 272649..272861 (+) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6M20_RS01315 267951..269207 - 1257 WP_149357467.1 IS256 family transposase -
D6M20_RS01320 269314..269481 - 168 WP_149357469.1 response regulator transcription factor -
D6M20_RS01325 269787..270581 + 795 WP_149357470.1 hypothetical protein -
D6M20_RS01330 270910..271944 - 1035 WP_149357471.1 diiron oxygenase -
D6M20_RS01335 272649..272861 + 213 WP_003943374.1 ribbon-helix-helix domain-containing protein Antitoxin
D6M20_RS01340 272858..273214 + 357 WP_003943396.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D6M20_RS01350 274874..275326 - 453 WP_149357472.1 hypothetical protein -
D6M20_RS01355 275612..275817 - 206 Protein_268 IS110 family transposase -
D6M20_RS01360 276418..276849 - 432 WP_125053505.1 hypothetical protein -
D6M20_RS01365 277586..277831 - 246 WP_021343300.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..448745 448745
- flank IS/Tn - - 267951..269207 1256


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-117)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-39)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12844.58 Da        Isoelectric Point: 6.8817

>T136599 WP_003943396.1 NZ_CP042915:272858-273214 [Rhodococcus qingshengii]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Download         Length: 357 bp

>T136599 NZ_CP056150:c4930817-4930401 [Klebsiella grimontii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTGCTGAAGCGCCT
GGAACAGGCGATGCTGCGCGGTGACCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACAGGCC
CGAAGGCCTCGCCGCGCCATATTCAACTGGTAGACGCGTTATGCGCGCGCCTCGATGCTGTCCTGCCCTTGGAACACTCC
GCGGTGGATGCCACGACGGACATCAAAGTGGCGCTGCGCCTCGTCGGGACGCCGATCGGCCCAAACGATACCGCGATAGC
CGGACACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATGTACGGGAGTTTGAGCGCGTGCCGGGCCTGATACTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7682.43 Da        Isoelectric Point: 4.1824

>AT136599 WP_003943374.1 NZ_CP042915:272649-272861 [Rhodococcus qingshengii]
MTTKFTISLPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVTADTE

Download         Length: 213 bp

>AT136599 NZ_CP042915:272649-272861 [Rhodococcus qingshengii]
ATGACCACGAAATTTACCATAAGTCTGCCTGACGAGGATGCGCACCTACTCGACGAGTATGTGCGTGATCACGGACTTCG
TGGCCGGTCGGCAGGAATTCGAGCCGCACTCCGCCTACTCTCGACGTCCGGACTCGAACGTGACTACGCTGCAGCGTTTG
CTGAAGGTGACGTAGATGAAGTGTGGGACGCTGTCACGGCAGACACCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2PUJ5

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2PWJ9

References