Detailed information of TA system TA136598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 253180..253715
• Replicon: plasmid unnamed2
• Accession: NZ_CP042915
• Organism: Rhodococcus qingshengii strain RL1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A069J573
• Locus tag: D6M20_RS01250
• Protein ID: WP_007733685.1
• Coordinates: 253180..253479 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8I0ZW53
• Locus tag: D6M20_RS01255
• Protein ID: WP_007733686.1
• Coordinates: 253479..253715 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6M20_RS01235	249302..250372	+	1071	WP_003941261.1	DUF1016 family protein	-
D6M20_RS01240	250504..251301	-	798	WP_149357465.1	helix-turn-helix domain-containing protein	-
D6M20_RS01245	251301..253151	-	1851	WP_149357466.1	hypothetical protein	-
D6M20_RS01250	253180..253479	-	300	WP_007733685.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D6M20_RS01255	253479..253715	-	237	WP_007733686.1	hypothetical protein	Antitoxin
D6M20_RS31675	253805..253969	+	165	WP_166813627.1	hypothetical protein	-
D6M20_RS01260	254148..254827	-	680	Protein_250	TetR/AcrR family transcriptional regulator	-
D6M20_RS01265	254883..255857	-	975	WP_030537938.1	DUF2637 domain-containing protein	-
D6M20_RS01270	256488..256802	-	315	WP_030537937.1	hypothetical protein	-
D6M20_RS01275	256799..257581	-	783	WP_007733691.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..448745	448745
	-	flank	IS/Tn	-	-	258414..259670	1256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10471.13 Da
• Isoelectric Point: 5.0079

>T136598 WP_007733685.1 NZ_CP042915:c253479-253180 [Rhodococcus qingshengii]
MRPIHEAKLDTVRPVLVLTREMVRPHLGTVTVAPITGEIRGLSTEVSVSVANGLSADAVVSCDHITTIPVSALGNIIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T136598 NZ_CP056150:4675121-4675339 [Klebsiella grimontii]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCCGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8738.77 Da
• Isoelectric Point: 6.4825

>AT136598 WP_007733686.1 NZ_CP042915:c253715-253479 [Rhodococcus qingshengii]
MSTQIAVRLPDEIVEFLDDEVREHRAASRASLVLRALERERRRQIAARDAEILSAATSEHDLSPLAHHAVNRQPTGST

Nucleotide

• Length: 237 bp

>AT136598 NZ_CP056150:4674720-4675094 [Klebsiella grimontii]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACTCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGTGCGGATCTGCAGAAATGGCGCAAATCTGGCAATCGGTT
ATTTCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A069J573