Detailed information of TA system TA136597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 94785..95303
• Replicon: plasmid unnamed2
• Accession: NZ_CP042915
• Organism: Rhodococcus qingshengii strain RL1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q6XN60
• Locus tag: D6M20_RS00445
• Protein ID: WP_011133429.1
• Coordinates: 94785..95087 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8E3P5W3
• Locus tag: D6M20_RS00450
• Protein ID: WP_064444958.1
• Coordinates: 95088..95303 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6M20_RS00435	91989..92786	-	798	WP_149357431.1	hypothetical protein	-
D6M20_RS00440	92786..94183	-	1398	WP_149357541.1	hypothetical protein	-
D6M20_RS00445	94785..95087	-	303	WP_011133429.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D6M20_RS00450	95088..95303	-	216	WP_064444958.1	hypothetical protein	Antitoxin
D6M20_RS00455	95443..95631	-	189	WP_082920751.1	hypothetical protein	-
D6M20_RS00465	95830..97125	-	1296	WP_149357432.1	AAA family ATPase	-
D6M20_RS00470	97196..98383	-	1188	WP_149357433.1	hypothetical protein	-
D6M20_RS00475	98380..98610	-	231	WP_149357434.1	hypothetical protein	-
D6M20_RS00480	98571..99809	-	1239	WP_149357435.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..448745	448745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10470.01 Da
• Isoelectric Point: 4.7317

>T136597 WP_011133429.1 NZ_CP042915:c95087-94785 [Rhodococcus qingshengii]
VRPIHDAQLGGLCPVLVLTRELVRPHLGTVTVAPIRSEGRGLSTELSLGEGNGVEDGSVVDCDGVTTIPVSALGVNRGFL
LPAQEVLLSRVLRSAFDLES

Nucleotide

• Length: 303 bp

>T136597 NZ_CP042915:c95087-94785 [Rhodococcus qingshengii]
GTGCGGCCGATTCACGACGCCCAGCTTGGAGGTCTTTGTCCGGTGTTGGTTCTGACGAGGGAATTGGTCCGGCCTCATCT
GGGGACGGTCACTGTTGCTCCCATTCGTTCGGAGGGGCGGGGGTTGTCGACGGAACTGAGTTTAGGCGAAGGCAACGGCG
TCGAGGACGGATCGGTTGTCGACTGCGACGGGGTGACGACGATCCCGGTGTCGGCACTCGGGGTGAATCGAGGTTTTCTC
TTGCCGGCTCAGGAAGTGTTGTTGAGCAGGGTTCTGCGATCAGCATTCGATCTCGAATCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7670.69 Da
• Isoelectric Point: 8.9167

>AT136597 WP_064444958.1 NZ_CP042915:c95303-95088 [Rhodococcus qingshengii]
MSTQIAVRLPEDIVAFLDSEVSGGRAKSRAQVVLRALEREQRRQLAVRDVELLSDGAGSDVNIAAAHGKKN

Nucleotide

• Length: 216 bp

>AT136597 NZ_CP042915:c95303-95088 [Rhodococcus qingshengii]
ATGAGCACGCAGATCGCGGTACGGCTACCCGAGGACATCGTGGCGTTTCTCGACAGCGAGGTCAGCGGTGGACGCGCGAA
AAGCCGTGCCCAAGTGGTGCTTCGAGCGTTGGAGCGTGAGCAGCGTCGGCAGCTGGCGGTCCGGGACGTGGAGCTCTTGT
CTGACGGCGCGGGTAGCGATGTGAATATCGCTGCCGCGCACGGAAAGAAGAACTGA

Structures

Toxin

• AlphaFold DB: Q6XN60