Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4290491..4291067 Replicon chromosome
Accession NZ_CP042903
Organism Escherichia coli strain CFSAN061761

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag CA899_RS20645 Protein ID WP_001297643.1
Coordinates 4290780..4291067 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag CA899_RS20640 Protein ID WP_000063160.1
Coordinates 4290491..4290793 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA899_RS20625 (4287087) 4287087..4289237 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
CA899_RS20630 (4289287) 4289287..4289490 + 204 WP_000467859.1 YbdD/YjiX family protein -
CA899_RS20635 (4289501) 4289501..4290457 + 957 WP_001297640.1 GTPase -
CA899_RS20640 (4290491) 4290491..4290793 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
CA899_RS20645 (4290780) 4290780..4291067 - 288 WP_001297643.1 BrnT family toxin Toxin
CA899_RS20650 (4291266) 4291266..4291962 - 697 Protein_4061 IS1 family transposase -
CA899_RS20655 (4292277) 4292277..4293509 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
CA899_RS20660 (4293550) 4293550..4294830 + 1281 WP_001037377.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4291266..4291475 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T136586 WP_001297643.1 NZ_CP042903:c4291067-4290780 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T136586 NZ_CP042903:c4291067-4290780 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT136586 WP_000063160.1 NZ_CP042903:c4290793-4290491 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT136586 NZ_CP042903:c4290793-4290491 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References