Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2501097..2501735 Replicon chromosome
Accession NZ_CP042903
Organism Escherichia coli strain CFSAN061761

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CA899_RS11860 Protein ID WP_000813794.1
Coordinates 2501559..2501735 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CA899_RS11855 Protein ID WP_001270286.1
Coordinates 2501097..2501513 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA899_RS11835 (2496249) 2496249..2497190 - 942 WP_001251304.1 ABC transporter permease -
CA899_RS11840 (2497191) 2497191..2498204 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
CA899_RS11845 (2498222) 2498222..2499367 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
CA899_RS11850 (2499612) 2499612..2501018 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
CA899_RS11855 (2501097) 2501097..2501513 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CA899_RS11860 (2501559) 2501559..2501735 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CA899_RS11865 (2501957) 2501957..2502187 + 231 WP_000494244.1 YncJ family protein -
CA899_RS11870 (2502279) 2502279..2504240 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CA899_RS11875 (2504313) 2504313..2504849 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CA899_RS11880 (2504902) 2504902..2506116 + 1215 WP_083583186.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T136580 WP_000813794.1 NZ_CP042903:c2501735-2501559 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T136580 NZ_CP042903:c2501735-2501559 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGTGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT136580 WP_001270286.1 NZ_CP042903:c2501513-2501097 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT136580 NZ_CP042903:c2501513-2501097 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References